휴먼 14-3-3 epsilon / YWHAE 단백질 (GST 태그)

  • Human 14-3-3 epsilon / YWHAE Protein (GST Tag) SDS-PAGE
데이터시트리뷰관련제품프로토콜
휴먼 YWHAE 단백질 제품 정보
동의어:14-3-3E,HEL2,KCIP-1,MDCR,MDS
단백질 구조:A DNA sequence encoding the human YWHAE (NP_006752.1) (Met 1-Gln 255) was fused with the GST tag at the N-terminus.
:Human
발현 숙주:E. coli
양식 및 배송:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
휴먼 YWHAE 단백질 품질 관리 검사
순도:> 92 % as determined by SDS-PAGE
내독소:Please contact us for more information.
안정성:Samples are stable for up to twelve months from date of receipt at -70℃
예상된 N 말단(N Terminal):Met
분자량:The recombinant human YWHAE/GST chimera consists of 487 amino acids and has a predicted molecular mass of 56 kDa. It migrates as an approxiamtely 50 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.25mM DTT, 25% glycerol, 0.5mM GSH, pH 7.5
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
휴먼 YWHAE 단백질 사용 가이드
보관:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
복원:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
휴먼 YWHAE Protein SDS-PAGE
Human 14-3-3 epsilon / YWHAE Protein (GST Tag) SDS-PAGE
Other YWHAE Recombinant Protein Products
10842-H09E
14-3-3 epsilon/YWHAE 연구배경

YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

휴먼 14-3-3 epsilon/YWHAE 참고자료
  • Ikeda M, et al. (2008) Identification of YWHAE, a gene encoding 14-3-3epsilon, as a possible susceptibility gene for schizophrenia. Hum Mol Genet. 17(20): 3212-22.
  • Mignon-Ravix C, et al. (2010) Deletion of YWHAE in a patient with periventricular heterotopias and pronounced corpus callosum hypoplasia. J Med Genet. 47(2): 132-6.
  • Nagamani SC, et al. (2009) Microdeletions including YWHAE in the Miller-Dieker syndrome region on chromosome 17p13.3 result in facial dysmorphisms, growth restriction, and cognitive impairment. J Med Genet. 46(12): 825-33.
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    Cat No: 10842-H09E-100
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    Datasheet & Documentation

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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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