Anti-14-3-3 eta Antibody

Cat: 10847-MM06

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Anti-14-3-3 eta Antibody (Mouse Monoclonal antibody) General Information

Product name
Anti-14-3-3 eta Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human 14-3-3 eta
No cross-reactivity in ELISA with
E.coli cell lysate
Immunogen
Recombinant Human 14-3-3 eta / YWHAH protein (Catalog#10847-H09E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Catalog#10847-H09E; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #06
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-14-3-3 eta Antibody (Mouse Monoclonal antibody) Validated Applications

Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 eta Antibody (Mouse Monoclonal antibody) Images

Human YWHAH Western blot (WB) 13293

Anti-YWHAH mouse monoclonal antibody at 1:500 dilution

Lane A: U937 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:30 kDa

Human YWHAH Immunoprecipitation(IP) 17677

YWHAH was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg k562 Whole Cell Lysate

2 µL anti-YWHAH mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-YWHAH mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Anti-14-3-3 eta Antibody Alternative Names

Anti-YWHA1 Antibody

14-3-3 eta Background Information

Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
References
  • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
  • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
  • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
  • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 eta Antibody Rabbit Polyclonal WB,ELISA,IP 10847-RP02
    Anti-14-3-3 eta Antibody Rabbit Polyclonal WB,IP 107196-T36
    Anti-14-3-3 eta Antibody Rabbit Polyclonal ELISA 10847-RP01
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