Anti-YWHAH rabbit polyclonal antibody at 1:500 dilution
Lane A: U937 Whole Cell Lysate
Lane B: 293T Whole Cell Lysate
Lane C: Jurkat Whole Cell Lysate
Lane D: K562 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:33 kDa
Observed band size:31 kDa
YWHAH was immunoprecipitated using:
Lane A:0.5 mg 293T Whole Cell Lysate
Lane B:0.5 mg Jurkat Whole Cell Lysate
Lane C:0.5 mg k562 Whole Cell Lysate
Lane D:0.5 mg NIH-3T3 Whole Cell Lysate2 µL anti-YWHAH rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-YWHAH rabbit polyclonal antibody,at 1:2000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 28 kDa
Observed band size: 28 kDa