휴먼 14-3-3 gamma/YWHAG Gene ORF cDNA clone in cloning vector

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    휴먼 YWHAG cDNA 클론 제품 정보
    Gene_bank_ref_id:NM_012479.3
    cDNA 크기:744bp
    cDNA 설명:Full length Clone DNA of Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide.
    유전자 동의어:14-3-3GAMMA,
    :Human
    벡터:pMD18-T Simple Vector
    Plasmid:pMD-YWHAG
    제한 사이트:
    태그 씨퀀스:
    염기서열 설명:Identical with the Gene Bank Ref. ID sequence except for the point mutation 450C/T not causing the amino acid variation.
    Sequencing primers:M13-47 and RV-M
    ( We provide with YWHAG qPCR primers for gene expression analysis, HP100743 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
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    Cat No: HG10846-M
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