휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone in cloning vector

    데이터시트리뷰관련제품프로토콜
    휴먼 YWHAS/SFN cDNA 클론 제품 정보
    Gene_bank_ref_id:NM_006142.3
    cDNA 크기:747bp
    cDNA 설명:Full length Clone DNA of Homo sapiens stratifin.
    유전자 동의어:YWHAS, SFN
    :Human
    벡터:pMD18-T Simple Vector
    Plasmid:pMD-SFN
    제한 사이트:
    태그 씨퀀스:
    염기서열 설명:Identical with the Gene Bank Ref. ID sequence except for the point mutation 746 G/A not causing the amino acid variation.
    Sequencing primers:M13-47 and RV-M
    ( We provide with SFN qPCR primers for gene expression analysis, HP100737 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone in cloning vector on other vectors
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG10838-ACG265260
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG10838-ACR265260
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그HG10838-ANG265260
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그HG10838-ANR265260
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, C-Flag 태그HG10838-CF229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, C-His 태그HG10838-CH229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, C-Myc 태그HG10838-CM229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, C-HA 태그HG10838-CY229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, C-Flag 태그HG10838-M-F229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-Flag 태그HG10838-NF229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-His 태그HG10838-NH229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-Myc 태그HG10838-NM229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-HA 태그HG10838-NY229890
    휴먼 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmidHG10838-UT229890
     발현 벡터에 대해 자세히 알아보기
    Product nameProduct name
    연구배경

    14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

    Size / Price
    Cat No: HG10838-M
    정가: 
    가격:      (You Save: )
    크기:
    수량:+-
    재고정보In Stock
    장바구니에 추가대량 주문 문의

    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    주의 : 모든 제품은 "연구 목적만을 위한 것이며 진단이나 치료에 사용하도록 의도되지 않았습니다".