휴먼 2B4 / SLAMF / CD244 단백질 (His 태그)

  • Human 2B4 / SLAMF / CD244 Protein (His Tag) SDS-PAGE
데이터시트리뷰관련제품프로토콜
휴먼 2B4/SLAMF4 단백질 제품 정보
동의어:2B4,NAIL,NKR2B4,Nmrk,SLAMF4
단백질 구조:A DNA sequence encoding the extracellular domain (Met 1-Arg 221) of human 2B4 (NP_057466.1) was expressed, with a polyhistidine tag at the C-terminus.
:Human
발현 숙주:Human Cells
양식 및 배송:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
휴먼 2B4/SLAMF4 단백질 품질 관리 검사
순도:> 95 % as determined by SDS-PAGE
생리활성:Measured by its binding ability in a functional ELISA . Immobilized human 2B4 at 2 μg/ml (100 μl/well) can bind human CD48. The EC50 of human CD48 is 0.39 μg/ml .
내독소:< 1.0 EU per μg of the protein as determined by the LAL method
안정성:Samples are stable for up to twelve months from date of receipt at -70℃
예상된 N 말단(N Terminal):Cys 22
분자량:The recombinant 2B4 comprises 211 amino acids and predicts a molecular mass of 23.8 kDa. As a result of glycosylation, the rh 2B4 protein migrates as an approximately 45-50 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
휴먼 2B4/SLAMF4 단백질 사용 가이드
보관:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
복원:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
휴먼 2B4/SLAMF4 Protein SDS-PAGE
Human 2B4 / SLAMF / CD244 Protein (His Tag) SDS-PAGE

2B4/SLAMF4 관련 경로

    Other 2B4/SLAMF4 Recombinant Protein Products
    10042-H08H
    2B4 / CD244 연구배경

    The CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.

    Immune Checkpoint
    Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies
    Immune Checkpoint Proteins
    Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

    Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    휴먼 2B4 / CD244 참고자료
  • Nakajima H, et al. (2000) 2B4: an NK cell activating receptor with unique specificity and signal transduction mechanism. Hum Immunol. 61(1): 39-43.
  • Chuang SS, et al. (2001) 2B4 (CD244)-mediated activation of cytotoxicity and IFN-gamma release in human NK cells involves distinct pathways. J Immunol. 167(11): 6210-6.
  • McNerney ME, et al. (2005) 2B4 (CD244) is a non-MHC binding receptor with multiple functions on natural killer cells and CD8+ T cells. Mol Immunol. 42(4): 489-94.
  • Mathew SO, et al. (2009) Functional role of human NK cell receptor 2B4 (CD244) isoforms. Eur J Immunol. 39(6): 1632-41.
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    Cat No: 10042-H08H-50
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    Datasheet & Documentation

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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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