5. How do I overcome inadequate or poor transfer problems?

5. How do I overcome inadequate or poor transfer problems?

Check to determine the size of the protein. If the protein is > 180 kDa, then you need to optimize transfer conditions by:
a. Using 20% MeOH.
b. Adding 0.05% SDS transfer buffer.
c. Increasing loading amounts of lysate.
d. Transfer for 1 hour at 1 A.

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