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쥐 AAMP Gene ORF cDNA clone in cloning vector

 AAMP cDNA 클론 제품 정보
cDNA 크기:1308
cDNA 설명:ORF Clone of Mus musculus angio-associated migratory protein DNA.
유전자 동의어:Aamp-rs, AU040907, MGC30660, Aamp
벡터:pMD18-T Simple Vector
제한 사이트:
태그 씨퀀스:
염기서열 설명:Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1-2 AT/TA not causing the amino acid variation.
Sequencing primers:( We provide with AAMP qPCR primers for gene expression analysis, MP200249 )
Antibiotic in E.coli:Ampicillin
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
보관:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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