All alanyl-tRNA synthetase reagents are produced in house and quality controlled, including 1 alanyl-tRNA synthetase Antibody, 15 alanyl-tRNA synthetase Gene, 1 alanyl-tRNA synthetase IPKit, 1 alanyl-tRNA synthetase Lysate, 1 alanyl-tRNA synthetase Protein, 1 alanyl-tRNA synthetase qPCR. All alanyl-tRNA synthetase reagents are ready to use.
Recombinant alanyl-tRNA synthetase proteins are expressed by Baculovirus-Insect Cells with fusion tags as C-His.
alanyl-tRNA synthetaseantibodies are validated with different applications, which are WB, ELISA, IHC-P, ICC/IF, IF, IP.
alanyl-tRNA synthetasecDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each alanyl-tRNA synthetase of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
Expression host: Baculovirus-Insect Cells
Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections.