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쥐 AARS / alanyl-tRNA synthetase 단백질 (His 태그)

데이터시트리뷰관련제품프로토콜
Mouse AARS 단백질 제품 정보
동의어:AI316495,C76919,sti
단백질 구조:A DNA sequence encoding the mouse AARS (Q8BGQ7) (Met 1-Asn 968) was fused with a polyhistidine tag at the C-terminus.
:Mouse
발현 숙주:Baculovirus-Insect Cells
양식 및 배송:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Mouse AARS 단백질 품질 관리 검사
순도:> 88 % as determined by SDS-PAGE
내독소:< 1.0 EU per μg of the protein as determined by the LAL method
안정성:Samples are stable for up to twelve months from date of receipt at -70℃
예상된 N 말단(N Terminal):Met 1
분자량:The secreted recombinant mouse AARS consists of 978 amino acids and has a calculated molecular mass of 108.3 kDa. The recombinant protein migrates as an approximately 105 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Mouse AARS 단백질 사용 가이드
보관:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
복원:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Mouse AARS Protein SDS-PAGE
Mouse AARS / alanyl-tRNA synthetase Protein (His Tag) SDS-PAGE
Other AARS Recombinant Protein Products
50384-M08B
AARS / alanyl-tRNA synthetase 연구배경

Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

Mouse AARS / alanyl-tRNA synthetase 참고자료
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
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    Cat No: 50384-M08B-100
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    • Mouse AARS / alanyl-tRNA synthetase Protein (His Tag) SDS-PAGE
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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