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휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone in cloning vector

    데이터시트리뷰관련제품프로토콜
    휴먼 AARS cDNA 클론 제품 정보
    Gene_bank_ref_id:NM_001605.2
    cDNA 크기:2907bp
    cDNA 설명:Full length Clone DNA of Homo sapiens alanyl-tRNA synthetase.
    유전자 동의어:AARS
    :Human
    벡터:pMD18-T Simple Vector
    Plasmid:pMD-AARS
    제한 사이트:
    태그 씨퀀스:
    염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:M13-47 and RV-M
    ( We provide with AARS qPCR primers for gene expression analysis, HP100828 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone in cloning vector on other vectors
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG10951-ACG383160
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG10951-ACR383160
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그HG10951-ANG383160
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그HG10951-ANR383160
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, C-Flag 태그HG10951-CF347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, C-His 태그HG10951-CH347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, C-Myc 태그HG10951-CM347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, C-HA 태그HG10951-CY347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmidHG10951-M-N347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, N-Flag 태그HG10951-NF347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, N-His 태그HG10951-NH347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, N-Myc 태그HG10951-NM347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmid, N-HA 태그HG10951-NY347790
    휴먼 AARS / alanyl-tRNA synthetase Gene ORF cDNA clone expression plasmidHG10951-UT347790
     발현 벡터에 대해 자세히 알아보기
    Product nameProduct name
    연구배경

    Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. 

    참고자료
  • Wakasugi K, et al. (1999) Two Distinct Cytokines Released from a Human Aminoacyl-tRNA Synthetase. Science. 284 (5411): 147-51.
  • Sokabe M, et al. (2009) The structure of alanyl-tRNA synthetase with editing domain. Proc Natl Acad Sci . 106 (27): 11028-33.
  • Skupinska M, et al. (2009) AARS--the etiological factor and the attractive target of many disorders. Postepy Biochem. 55 (4): 373-84.
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    Cat No: HG10951-M
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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