ABHD10 (Protein|Antibody|cDNA Clone|ELISA Kit)

All ABHD10 reagents are produced in house and quality controlled, including 32 ABHD10 Gene, 1 ABHD10 Lysate, 1 ABHD10 Protein, 1 ABHD10 qPCR. All ABHD10 reagents are ready to use.

Recombinant ABHD10 proteins are expressed by Baculovirus-Insect Cells with fusion tags as N-His.

ABHD10 cDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each ABHD10 of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.

ABHD10 Protein (1)

Species

ABHD10 Protein, Human, Recombinant (aa 53-306, His Tag)

14439-H07B

Expression host: Baculovirus-Insect Cells

Human ABHD10 Protein 10598
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ABHD10 cDNA Clone (32)

Rat
Human
Mouse

ABHD10 qPCR Primer (1)

ABHD10 Lysate (1)

Mycophenolic acid (MPA), the active metabolite of the immunosuppressant mycophenolate mofetil (MMF), is primarily metabolized by glucuronidation to a phenolic glucuronide (MPAG) and an acyl glucuronide (AcMPAG). It is known that AcMPAG, which may be an immunotoxic metabolite, is deglucuronidated in human liver. AcMPAG deglucuronidation activity was detected in both human liver cytosol (HLC) and microsomes (HLM). By purification from HLC with column chromatographic purification steps, the enzyme responsible for AcMPAG deglucuronidationis identified as α/β hydrolase domain containing 1 (ABHD1). Recombinant ABHD1 expressed in Sf9 cells efficiently deglucuronidated AcMPAG with a K(m) value of 1.7 ± 1.2 μM, which was similar to those in HLM, HLC, and human liver homogenates (HLH). Immunoblot analysis revealed ABHD1 protein expression in both HLC and HLM. The AcMPAG deglucuronidation by recombinant ABHD1, HLC, and HLH were potently inhibited by AgNO(3), CdCl(2), CuCl(2), PMSF, bis-p-nitrophenylphosphate, and DTNB. The CL(int) value of AcMPAG formation from MPA, which was catalyzed by human UGT2B7, in HLH was increased by 1.8-fold in the presence of PMSF. Thus, human ABHD1 would affect the formation of AcMPAG, the immunotoxic metabolite.
Protein
Gene
qPCR
Lysate
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