ABHD14B Protein, Human, Recombinant (His Tag)

Cat: 13913-H07E

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ABHD14B Protein, Human, Recombinant (His Tag) Product Information

Purity
> 96 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the mature form of human ABHD14B (Q96IU4-1) (Met1-Gln210) was expressed with a polyhistide tag at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
His
Molecule Mass
The recombinant human ABHD14B consists of 225 amino acids and predicts a molecular mass of 24.2 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, 20% glycerol, 50mM Arg, 0.1% Tween20, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

ABHD14B Protein, Human, Recombinant (His Tag) in SDS-PAGE

Human ABHD14B Protein 10483

ABHD14B Protein, Human, Recombinant (His Tag) Alternative Names

CIB Protein, Human;HEL-S-299 Protein, Human

ABHD14B Background Information

ABHD14B belongs to the AB hydrolase superfamily, ABHD14 family. It can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocyte, heart, placenta, lung, liver, skeletal muscle, pancreas and kidney. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) and may interact with TAF1. It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. ABHD14B contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing gene subfamily is comprised of 15 mostly uncharacterized members.

Full Name
abhydrolase domain containing 14B
References
  • Mehrle A, et al. (2006) The LIFEdb database in 2006. Nucleic Acids Res. 34:D415-8.
  • Wan D, et al. (2004) Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc Natl Acad Sci. 101(44):15724-9.
  • Wiemann S, et al. (2004) From ORFeome to biology: a functional genomics pipeline. Genome Res. 14 (10B):2136-44.
  • Other ABHD14B Proteins

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