휴먼(Human) ACE 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

ACE cDNA Clone Datasheet

ACE cDNA Clone Product Information

• Gene Bank Ref.ID: NM_000789.2
• cDNA Size: 3921
• Gene Synonym: DCP; ACE1; DCP1; CD143; MGC26566; ACE
• Source: Human

ACE cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for five point mutations: 1215 C/T, 2193 A/G, 2328 G/A and 3387 T/C not causing the amino acid variation and 3853 C/T resulting in the amino acid His substitution by Tyr.

ACE cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ACE cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

ACE cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGCCG CCTCGGGCCG CCGGGGGCCG GGGCTGCTGC TGCCGCTGCC GCTGCTGTTG
61       CTGCTGCCGC CGCAGCCCGC CCTGGCGTTG GACCCCGGGC TGCAGCCCGG CAACTTTTCT
121      GCTGACGAGG CCGGGGCGCA GCTCTTCGCG CAGAGCTACA ACTCCAGCGC CGAACAGGTG
181      CTGTTCCAGA GCGTGGCCGC CAGCTGGGCG CACGACACCA ACATCACCGC GGAGAATGCA
241      AGGCGCCAGG AGGAAGCAGC CCTGCTCAGC CAGGAGTTTG CGGAGGCCTG GGGCCAGAAG
301      GCCAAGGAGC TGTATGAACC GATCTGGCAG AACTTCACGG ACCCGCAGCT GCGCAGGATC
361      ATCGGAGCTG TGCGCACCCT GGGCTCTGCC AACCTGCCCC TGGCTAAGCG GCAGCAGTAC
421      AACGCCCTGC TAAGCAACAT GAGCAGGATC TACTCCACCG CCAAGGTCTG CCTCCCCAAC
481      AAGACTGCCA CCTGCTGGTC CCTGGACCCA GATCTCACCA ACATCCTGGC TTCCTCGCGA
541      AGCTACGCCA TGCTCCTGTT TGCCTGGGAG GGCTGGCACA ACGCTGCGGG CATCCCGCTG
601      AAACCGCTGT ACGAGGATTT CACTGCCCTC AGCAATGAAG CCTACAAGCA GGACGGCTTC
661      ACAGACACGG GGGCCTACTG GCGCTCCTGG TACAACTCCC CCACCTTCGA GGACGATCTG
721      GAACACCTCT ACCAACAGCT AGAGCCCCTC TACCTGAACC TCCATGCCTT CGTCCGCCGC
781      GCACTGCATC GCCGATACGG AGACAGATAC ATCAACCTCA GGGGACCCAT CCCTGCTCAT
841      CTGCTGGGAG ACATGTGGGC CCAGAGCTGG GAAAACATCT ACGACATGGT GGTGCCTTTC
901      CCAGACAAGC CCAACCTCGA TGTCACCAGT ACTATGCTGC AGCAGGGCTG GAACGCCACG
961      CACATGTTCC GGGTGGCAGA GGAGTTCTTC ACCTCCCTGG AGCTCTCCCC CATGCCTCCC
1021     GAGTTCTGGG AAGGGTCGAT GCTGGAGAAG CCGGCCGACG GGCGGGAAGT GGTGTGCCAC
1081     GCCTCGGCTT GGGACTTCTA CAACAGGAAA GACTTCAGGA TCAAGCAGTG CACACGGGTC
1141     ACGATGGACC AGCTCTCCAC AGTGCACCAT GAGATGGGCC ATATACAGTA CTACCTGCAG
1201     TACAAGGATC TGCCTGTCTC CCTGCGTCGG GGGGCCAACC CCGGCTTCCA TGAGGCCATT
1261     GGGGACGTGC TGGCGCTCTC GGTCTCCACT CCTGAACATC TGCACAAAAT CGGCCTGCTG
1321     GACCGTGTCA CCAATGACAC GGAAAGTGAC ATCAATTACT TGCTAAAAAT GGCACTGGAA
1381     AAAATTGCCT TCCTGCCCTT TGGCTACTTG GTGGACCAGT GGCGCTGGGG GGTCTTTAGT
1441     GGGCGTACCC CCCCTTCCCG CTACAACTTC GACTGGTGGT ATCTTCGAAC CAAGTATCAG
1501     GGGATCTGTC CTCCTGTTAC CCGAAACGAA ACCCACTTTG ATGCTGGAGC TAAGTTTCAT
1561     GTTCCAAATG TGACACCATA CATCAGGTAC TTTGTGAGTT TTGTCCTGCA GTTCCAGTTC
1621     CATGAAGCCC TGTGCAAGGA GGCAGGCTAT GAGGGCCCAC TGCACCAGTG TGACATCTAC
1681     CGGTCCACCA AGGCAGGGGC CAAGCTCCGG AAGGTGCTGC AGGCTGGCTC CTCCAGGCCC
1741     TGGCAGGAGG TGCTGAAGGA CATGGTCGGC TTAGATGCCC TGGATGCCCA GCCGCTGCTC
1801     AAGTACTTCC AGCCAGTCAC CCAGTGGCTG CAGGAGCAGA ACCAGCAGAA CGGCGAGGTC
1861     CTGGGCTGGC CCGAGTACCA GTGGCACCCG CCGTTGCCTG ACAACTACCC GGAGGGCATA
1921     GACCTGGTGA CTGATGAGGC TGAGGCCAGC AAGTTTGTGG AGGAATATGA CCGGACATCC
1981     CAGGTGGTGT GGAACGAGTA TGCCGAGGCC AACTGGAACT ACAACACCAA CATCACCACA
2041     GAGACCAGCA AGATTCTGCT GCAGAAGAAC ATGCAAATAG CCAACCACAC CCTGAAGTAC
2101     GGCACCCAGG CCAGGAAGTT TGATGTGAAC CAGTTGCAGA ACACCACTAT CAAGCGGATC
2161     ATAAAGAAGG TTCAGGACCT AGAACGGGCA GCGCTGCCTG CCCAGGAGCT GGAGGAGTAC
2221     AACAAGATCC TGTTGGATAT GGAAACCACC TACAGCGTGG CCACTGTGTG CCACCCGAAT
2281     GGCAGCTGCC TGCAGCTCGA GCCAGATCTG ACGAATGTGA TGGCCACATC CCGGAAATAT
2341     GAAGACCTGT TATGGGCATG GGAGGGCTGG CGAGACAAGG CGGGGAGAGC CATCCTCCAG
2401     TTTTACCCGA AATACGTGGA ACTCATCAAC CAGGCTGCCC GGCTCAATGG CTATGTAGAT
2461     GCAGGGGACT CGTGGAGGTC TATGTACGAG ACACCATCCC TGGAGCAAGA CCTGGAGCGG
2521     CTCTTCCAGG AGCTGCAGCC ACTCTACCTC AACCTGCATG CCTACGTGCG CCGGGCCCTG
2581     CACCGTCACT ACGGGGCCCA GCACATCAAC CTGGAGGGGC CCATTCCTGC TCACCTGCTG
2641     GGGAACATGT GGGCGCAGAC CTGGTCCAAC ATCTATGACT TGGTGGTGCC CTTCCCTTCA
2701     GCCCCCTCGA TGGACACCAC AGAGGCTATG CTAAAGCAGG GCTGGACGCC CAGGAGGATG
2761     TTTAAGGAGG CTGATGATTT CTTCACCTCC CTGGGGCTGC TGCCCGTGCC TCCTGAGTTC
2821     TGGAACAAGT CGATGCTGGA GAAGCCAACC GACGGGCGGG AGGTGGTCTG CCACGCCTCG
2881     GCCTGGGACT TCTACAACGG CAAGGACTTC CGGATCAAGC AGTGCACCAC CGTGAACTTG
2941     GAGGACCTGG TGGTGGCCCA CCACGAAATG GGCCACATCC AGTATTTCAT GCAGTACAAA
3001     GACTTACCTG TGGCCTTGAG GGAGGGTGCC AACCCCGGCT TCCATGAGGC CATTGGGGAC
3061     GTGCTAGCCC TCTCAGTGTC TACGCCCAAG CACCTGCACA GTCTCAACCT GCTGAGCAGT
3121     GAGGGTGGCA GCGACGAGCA TGACATCAAC TTTCTGATGA AGATGGCCCT TGACAAGATC
3181     GCCTTTATCC CCTTCAGCTA CCTCGTCGAT CAGTGGCGCT GGAGGGTATT TGATGGAAGC
3241     ATCACCAAGG AGAACTATAA CCAGGAGTGG TGGAGCCTCA GGCTGAAGTA CCAGGGCCTC
3301     TGCCCCCCAG TGCCCAGGAC TCAAGGTGAC TTTGACCCAG GGGCCAAGTT CCACATTCCT
3361     TCTAGCGTGC CTTACATCAG GTACTTCGTC AGCTTCATCA TCCAGTTCCA GTTCCACGAG
3421     GCACTGTGCC AGGCAGCTGG CCACACGGGC CCCCTGCACA AGTGTGACAT CTACCAGTCC
3481     AAGGAGGCCG GGCAGCGCCT GGCGACCGCC ATGAAGCTGG GCTTCAGTAG GCCGTGGCCG
3541     GAAGCCATGC AGCTGATCAC GGGCCAGCCC AACATGAGCG CCTCGGCCAT GTTGAGCTAC
3601     TTCAAGCCGC TGCTGGACTG GCTCCGCACG GAGAACGAGC TGCATGGGGA GAAGCTGGGC
3661     TGGCCGCAGT ACAACTGGAC GCCGAACTCC GCTCGCTCAG AAGGGCCCCT CCCAGACAGC
3721     GGCCGCGTCA GCTTCCTGGG CCTGGACCTG GATGCGCAGC AGGCCCGCGT GGGCCAGTGG
3781     CTGCTGCTCT TCCTGGGCAT CGCCCTGCTG GTAGCCACCC TGGGCCTCAG CCAGCGGCTC
3841     TTCAGCATCC GCTACCGCAG CCTCCACCGG CACTCCCACG GGCCCCAGTT CGGCTCCGAG
3901     GTGGAGCTGA GACACTCCTG A

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).