ACOX1: ACOX1 Protein | ACOX1 Antibody

ACOX1 Background

Peroxisomal acyl-coenzyme A oxidase 1(ACOX1 or AOX) is the first enzyme of the fatty acid beta-oxidation pathway and belongs to the Acyl-CoA oxidase family. Human liver peroxisomes contain two acyl-CoA oxidases, namely, palmitoyl-CoA oxidase (ACOX1/AOX) and a branched chain acyl-CoA oxidase. The palmitoyl-CoA oxidase (ACOX1/AOX) oxidizes the CoA esters of straight chain fatty acids and prostaglandins and donates electrons directly to molecular oxygen, thereby producing H2O2. Human ACOX1/AOX is a protein of 661-amino acids, including the carboxyl-terminal sequence(Ser-Lys-Leu) known as a minimal peroxisome-targeting signal. Human ACOX1/AOX, the first and rate-limiting enzyme of the peroxisomal β-oxidation pathway, has two isoforms including ACOX1a and ACOX1b, transcribed from a single gene. The human ACOX1b isoform is more effective than the ACOX1a isoform in reversing the Acox1 null phenotype in the mouse partly because of the Substrate utilization differences.

Reference for ACOX1

  • Vluggens A, et al. (2010) Functional significance of the two ACOX1 isoforms and their crosstalks with PPAR alpha and RXR alpha. Laboratory Investigation. 90: 696-708.
  • Chu R, et al. (1995) Overexpression and characterization of the human peroxisomal acyl-CoA oxidase in insect cells. J Biol Chem. 270 (9): 4908-15.
  • Aoyama T, et al. (1994) Molecular cloning and functional expression of a human peroxisomal acyl-coenzyme A oxidase. Biochem Biophys Res Commun. 198 (3): 1113-8.

ACOX1 Protein

ACOX1 protein function

Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA. {ECO:0000269|PubMed:17458872, ECO:0000269|PubMed:17603022}.

ACOX1 protein expression

Tissue specificity

Widely expressed with highest levels of isoform 1 and isoform 2 detected in testis. Isoform 1 is expressed at higher levels than isoform 2 in liver and kidney while isoform 2 levels are higher in brain, lung, muscle, white adipose tissue and testis. Levels are almost equal in heart.

ACOX1 protein sequence

This sequence information is just for reference only.From Uniport

  • Length
  • Mass (Da)

ACOX1 Antibody

There are 7 ACOX1 antibodies which are validated in multiple tissues with various applications, including IHC-P, ELISA, ICC/IF, IF. There are 4 ACOX1 antibody for IHC-P, 4 ACOX1 antibody for ELISA, 1 ACOX1 antibody for ICC/IF, 1 ACOX1 antibody for IF. Among all these ACOX1 antibodies, there are 1 anti-ACOX1 mouse monoclonal antibodies , 3 anti-ACOX1 rabbit monoclonal antibodies , 3 anti-ACOX1 rabbit polyclonal antibodies . All the ACOX1 anbodies are produced in house and all are in stock. ACOX1 antibody customerized service is available.

ACOX1 Gene

ACOX1 gene / cDNA is a protein-coding gene which located on 17q25.1. The ACOX1 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, A.thaliana, rice, and frog.238 organisms have orthologs with human gene ACOX1.