휴먼(Human) AGER 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

AGER cDNA Clone Datasheet

AGER cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001136.3
• cDNA Size: 1215
• Gene Synonym: RAGE; MGC22357; AGER
• Source: Human

AGER cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens advanced glycosylation end product-specific receptor, isform 1 DNA.
• Vector: pMD18-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

AGER cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

AGER cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

AGER cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCCG GAACAGCAGT TGGAGCCTGG GTGCTGGTCC TCAGTCTGTG GGGGGCAGTA
61       GTAGGTGCTC AAAACATCAC AGCCCGGATT GGCGAGCCAC TGGTGCTGAA GTGTAAGGGG
121      GCCCCCAAGA AACCACCCCA GCGGCTGGAA TGGAAACTGA ACACAGGCCG GACAGAAGCT
181      TGGAAGGTCC TGTCTCCCCA GGGAGGAGGC CCCTGGGACA GTGTGGCTCG TGTCCTTCCC
241      AACGGCTCCC TCTTCCTTCC GGCTGTCGGG ATCCAGGATG AGGGGATTTT CCGGTGCCAG
301      GCAATGAACA GGAATGGAAA GGAGACCAAG TCCAACTACC GAGTCCGTGT CTACCAGATT
361      CCTGGGAAGC CAGAAATTGT AGATTCTGCC TCTGAACTCA CGGCTGGTGT TCCCAATAAG
421      GTGGGGACAT GTGTGTCAGA GGGAAGCTAC CCTGCAGGGA CTCTTAGCTG GCACTTGGAT
481      GGGAAGCCCC TGGTGCCTAA TGAGAAGGGA GTATCTGTGA AGGAACAGAC CAGGAGACAC
541      CCTGAGACAG GGCTCTTCAC ACTGCAGTCG GAGCTAATGG TGACCCCAGC CCGGGGAGGA
601      GATCCCCGTC CCACCTTCTC CTGTAGCTTC AGCCCAGGCC TTCCCCGACA CCGGGCCTTG
661      CGCACAGCCC CCATCCAGCC CCGTGTCTGG GAGCCTGTGC CTCTGGAGGA GGTCCAATTG
721      GTGGTGGAGC CAGAAGGTGG AGCAGTAGCT CCTGGTGGAA CCGTAACCCT GACCTGTGAA
781      GTCCCTGCCC AGCCCTCTCC TCAAATCCAC TGGATGAAGG ATGGTGTGCC CTTGCCCCTT
841      CCCCCCAGCC CTGTGCTGAT CCTCCCTGAG ATAGGGCCTC AGGACCAGGG AACCTACAGC
901      TGTGTGGCCA CCCATTCCAG CCACGGGCCC CAGGAAAGCC GTGCTGTCAG CATCAGCATC
961      ATCGAACCAG GCGAGGAGGG GCCAACTGCA GGCTCTGTGG GAGGATCAGG GCTGGGAACT
1021     CTAGCCCTGG CCCTGGGGAT CCTGGGAGGC CTGGGGACAG CCGCCCTGCT CATTGGGGTC
1081     ATCTTGTGGC AAAGGCGGCA ACGCCGAGGA GAGGAGAGGA AGGCCCCAGA AAACCAGGAG
1141     GAAGAGGAGG AGCGTGCAGA ACTGAATCAG TCGGAGGAAC CTGAGGCAGG CGAGAGTAGT
1201     ACTGGAGGGC CTTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).