휴먼(Human) AGTR1/AT1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_000685.4

cDNA Size:   1080

Synonym:  AT1, AG2S, AT1B, AT1R, AT2R1, HAT1R, AGTR1A, AGTR1B, AT2R1A, AT2R1B

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens angiotensin II receptor, type 1, transcript variant 1 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the 573 T/C mutation not causing amino acid variation.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGATTCTCA ACTCTTCTAC TGAAGATGGT ATTAAAAGAA TCCAAGATGA TTGTCCCAAA
61       GCTGGAAGGC ATAATTACAT ATTTGTCATG ATTCCTACTT TATACAGTAT CATCTTTGTG
121      GTGGGAATAT TTGGAAACAG CTTGGTGGTG ATAGTCATTT ACTTTTATAT GAAGCTGAAG
181      ACTGTGGCCA GTGTTTTTCT TTTGAATTTA GCACTGGCTG ACTTATGCTT TTTACTGACT
241      TTGCCACTAT GGGCTGTCTA CACAGCTATG GAATACCGCT GGCCCTTTGG CAATTACCTA
301      TGTAAGATTG CTTCAGCCAG CGTCAGTTTC AACCTGTACG CTAGTGTGTT TCTACTCACG
361      TGTCTCAGCA TTGATCGATA CCTGGCTATT GTTCACCCAA TGAAGTCCCG CCTTCGACGC
421      ACAATGCTTG TAGCCAAAGT CACCTGCATC ATCATTTGGC TGCTGGCAGG CTTGGCCAGT
481      TTGCCAGCTA TAATCCATCG AAATGTATTT TTCATTGAGA ACACCAATAT TACAGTTTGT
541      GCTTTCCATT ATGAGTCCCA AAATTCAACC CTCCCGATAG GGCTGGGCCT GACCAAAAAT
601      ATACTGGGTT TCCTGTTTCC TTTTCTGATC ATTCTTACAA GTTATACTCT TATTTGGAAG
661      GCCCTAAAGA AGGCTTATGA AATTCAGAAG AACAAACCAA GAAATGATGA TATTTTTAAG
721      ATAATTATGG CAATTGTGCT TTTCTTTTTC TTTTCCTGGA TTCCCCACCA AATATTCACT
781      TTTCTGGATG TATTGATTCA ACTAGGCATC ATACGTGACT GTAGAATTGC AGATATTGTG
841      GACACGGCCA TGCCTATCAC CATTTGTATA GCTTATTTTA ACAATTGCCT GAATCCTCTT
901      TTTTATGGCT TTCTGGGGAA AAAATTTAAA AGATATTTTC TCCAGCTTCT AAAATATATT
961      CCCCCAAAAG CCAAATCCCA CTCAAACCTT TCAACAAAAA TGAGCACGCT TTCCTACCGC
1021     CCCTCAGATA ATGTAAGCTC ATCCACCAAG AAGCCTGCAC CATGTTTTGA GGTTGAGTGA

Quality Control:   cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).