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휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone in cloning vector

데이터시트리뷰관련제품프로토콜
Human AK4/AK3L1 cDNA 클론 제품 정보
Gene_bank_ref_id:BC016180
cDNA 크기:672bp
cDNA 설명:Full length Clone DNA of Homo sapiens adenylate kinase 4.
유전자 동의어:AK3, AK3L1, AK3L2
:Human
벡터:pGEM-T Vector
Plasmid:pGEM-AK3L1
제한 사이트:
태그 씨퀀스:
염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:SP6 and T7 or M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
pGEM-T Vector Information

The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

pGEM-T Simple Usage Suggestion:

The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

Vector Sequence Download
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone in cloning vector on other vectors
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그HG12406-ACG265260
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그HG12406-ACR265260
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그HG12406-ANG265260
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그HG12406-ANR265260
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-Flag 태그HG12406-CF229890
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-His 태그HG12406-CH229890
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-Myc 태그HG12406-CM229890
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, C-HA 태그HG12406-CY229890
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Flag 태그HG12406-NF229890
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-His 태그HG12406-NH229890
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-Myc 태그HG12406-NM229890
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmid, N-HA 태그HG12406-NY229890
휴먼 AK4 / Adenylate Kinase 4 / AK3L1 Gene ORF cDNA clone expression plasmidHG12406-UT229890
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연구배경

Adenylate kinase isoenzyme 4, mitochondrial, also known as ATP-AMP transphosphorylase, Adenylate kinase 3-like, AK4 and AK3L1, is a member the adenylate kinase family. AK4 / AK3L1 is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. AK4 / AK3L1 catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It may also be active with GTP. Adenylate kinase 4 ( AK4 / AK3L1 ) is a unique member with no enzymatic activity in the adenylate kinase (AK) family although it shares high sequence homology with other AKs. It remains unclear what physiological function AK4 might play or why it is enzymatically inactive. AK4 / AK3L1 retains the capability of binding nucleotides. It has a glutamine residue instead of a key arginine residue in the active site well conserved in other AKs. The enzymatically inactive AK4 is a stress responsive protein critical to cell survival and proliferation. AK4 / AK3L1 is likely that the interaction with the mitochondrial inner membrane protein ANT is important for AK4 to exert the protective benefits to cells under stress. AK4 / AK3L1 also acts on the specific mechanism of energy metabolism rather than control of the homeostasis of the ADP pool ubiquitously.

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Cat No: HG12406-G
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