휴먼(Human) AKR1A1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

AKR1A1 cDNA Clone Datasheet

AKR1A1 cDNA Clone Product Information

• Gene Bank Ref.ID: BC000670.2
• cDNA Size: 978
• Gene Synonym: ALR; ARM; DD3; ALDR1; MGC1380; MGC12529; AKR1A1
• Source: Human

AKR1A1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens aldo-keto reductase family 1, member A1 (aldehyde reductase) DNA.
• Vector: pMD18-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

AKR1A1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

AKR1A1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

AKR1A1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGCTT CCTGTGTTCT ACTGCACACT GGGCAGAAGA TGCCTCTGAT TGGTCTGGGT
61       ACCTGGAAGA GTGAGCCTGG TCAGGTAAAA GCAGCTGTTA AGTATGCCCT TAGCGTAGGC
121      TACCGCCACA TTGATTGTGC TGCTATCTAC GGCAATGAGC CTGAGATTGG GGAGGCCCTG
181      AAGGAGGACG TGGGACCAGG CAAGGCGGTG CCTCGGGAGG AGCTGTTTGT GACATCCAAG
241      CTGTGGAACA CCAAGCACCA CCCCGAGGAT GTGGAGCCTG CCCTCCGGAA GACTCTGGCT
301      GACCTCCAGC TGGAGTATCT GGACCTGTAC CTGATGCACT GGCCTTATGC CTTTGAGCGG
361      GGAGACAACC CCTTCCCCAA GAATGCTGAT GGGACTATAT GCTACGACTC CACCCACTAC
421      AAGGAGACTT GGAAGGCTCT GGAGGCACTG GTGGCTAAGG GGCTGGTGCA GGCGCTGGGC
481      CTGTCCAACT TCAACAGTCG GCAGATTGAT GACATACTCA GTGTGGCCTC CGTGCGTCCA
541      GCTGTCTTGC AGGTGGAATG CCACCCATAC TTGGCTCAAA ATGAGCTAAT TGCCCACTGC
601      CAAGCACGTG GCCTGGAGGT AACTGCTTAT AGCCCTTTGG GCTCCTCTGA TCGTGCATGG
661      CGTGATCCTG ATGAGCCTGT CCTGCTGGAG GAACCAGTAG TCCTGGCATT GGCTGAAAAG
721      TATGGCCGAT CTCCAGCTCA GATCTTGCTC AGGTGGCAGG TCCAGCGGAA AGTGATCTGC
781      ATCCCCAAAA GTATCACTCC TTCTCGAATC CTTCAGAACA TCAAGGTGTT TGACTTCACC
841      TTTAGCCCAG AAGAGATGAA GCAGCTAAAT GCCCTGAACA AAAATTGGAG ATATATTGTG
901      CCTATGCTTA CGGTGGATGG GAAGAGAGTC CCAAGGGATG CAGGGCATCC TCTGTACCCC
961      TTTAATGACC CGTACTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).