휴먼(Human) ASMTL 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

ASMTL cDNA Clone Datasheet

ASMTL cDNA Clone Product Information

• Gene Bank Ref.ID: NM_004192.2
• cDNA Size: 1866
• Gene Synonym: ASTML; ASMTLX; ASMTLY; ASMTL
• Source: Human

ASMTL cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens acetylserotonin O-methyltransferase-like DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 6 G/A, 276 A/G not causing the amino acid variation.

ASMTL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

ASMTL cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

ASMTL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTACTGT GCCCGGTGAT TGGGAAGCTG CTGCACAAGC GCGTGGTGCT GGCCAGCGCC
61       TCCCCACGCC GTCAGGAGAT CCTCAGCAAC GCGGGTCTCA GGTTTGAGGT GGTCCCCTCC
121      AAGTTTAAAG AGAAGCTGGA CAAAGCCTCC TTCGCTACTC CGTATGGGTA CGCCATGGAG
181      ACCGCCAAGC AGAAGGCCCT GGAGGTGGCC AACCGGCTGT ACCAGAAAGA CCTGCGGGCC
241      CCCGACGTGG TCATTGGAGC GGACACGATC GTGACGGTCG GGGGGCTGAT TCTGGAGAAG
301      CCGGTGGACA AGCAGGACGC CTACAGGATG CTGTCCCGGT TGAGTGGGAG AGAACACAGC
361      GTGTTCACAG GTGTCGCGAT CGTCCACTGC TCCAGCAAAG ACCATCAGCT GGACACCAGG
421      GTCTCGGAAT TCTACGAGGA AACGAAGGTG AAGTTCTCGG AGCTGTCCGA GGAGCTGCTC
481      TGGGAATACG TCCACAGCGG GGAGCCCATG GACAAAGCTG GCGGCTACGG GATCCAGGCC
541      CTGGGCGGCA TGCTGGTGGA GTCCGTACAC GGGGACTTTC TGAACGTGGT GGGATTCCCG
601      CTGAACCACT TCTGCAAGCA GCTGGTGAAG CTCTACTACC CGCCCCGTCC GGAGGACCTG
661      CGGCGGAGTG TCAAGCACGA CTCCATCCCG GCCGCGGACA CCTTCGAAGA CCTCAGTGAC
721      GTGGAGGGGG GCGGCTCGGA GCCCACTCAG AGGGACGCGG GCAGCCGCGA TGAGAAGGCC
781      GAGGCGGGAG AGGCGGGACA GGCCACGGCA GAGGCTGAGT GTCACAGGAC TCGGGAGACC
841      CTGCCTCCGT TCCCGACACG CCTCCTGGAG CTGATTGAGG GCTTTATGCT ATCCAAGGGC
901      CTGCTCACCG CTTGCAAACT GAAGGTGTTC GATTTGTTAA AAGATGAAGC ACCCCAGAAG
961      GCTGCGGATA TTGCCAGCAA AGTGGACGCC TCTGCGTGTG GAATGGAGAG GCTTCTGGAC
1021     ATCTGTGCTG CCATGGGGCT CCTGGAGAAG ACAGAGCAAG GTTACAGTAA CACAGAGACA
1081     GCGAACGTCT ACCTGGCATC GGATGGCGAA TACTCTCTGC ACGGCTTCAT CATGCACAAT
1141     AATGACCTCA CATGGAACCT CTTTACATAC CTGGAGTTTG CCATCCGAGA GGGAACAAAC
1201     CAGCACCACA GGGCGTTGGG GAAGAAGGCG GAAGATCTGT TCCAGGATGC GTACTACCAG
1261     AGCCCGGAGA CGCGGCTGAG GTTCATGCGG GCCATGCACG GCATGACGAA GCTGACTGCG
1321     TGCCAGGTGG CCACGGCCTT CAATCTGTCC CGCTTCTCCT CCGCCTGCGA CGTGGGAGGC
1381     TGCACGGGTG CACTGGCCCG AGAGCTGGCC CGTGAGTACC CTCGTATGCA GGTGACTGTG
1441     TTTGACCTCC CAGACATTAT CGAGCTGGCC GCCCACTTCC AACCCCCCGG ACCGCAGGCA
1501     GTGCAGATCC ACTTCGCAGC AGGTGACTTT TTCAGGGACC CCCTCCCCAG CGCTGAGCTG
1561     TACGTCCTGT GCCGGATCCT GCATGACTGG CCAGACGACA AAGTCCACAA GTTACTCAGC
1621     AGGGTCGCCG AGAGCTGCAA GCCAGGGGCC GGCCTGCTGC TGGTGGAGAC GCTCCTGGAT
1681     GAGGAGAAGA GGGTGGCGCA GCGCGCCCTG ATGCAGTCAC TGAACATGCT GGTGCAGACT
1741     GAAGGCAAGG AGCGGAGCCT GGGCGAGTAT CAGTGCTTGC TGGAGCTGCA CGGCTTCCAC
1801     CAGGTGCAGG TGGTGCACTT GGGGGGTGTC CTGGATGCCA TCTTGGCCAC CAAAGTGGCC
1861     CCCTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).