마우스(Mouse) AXL 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

AXL cDNA Clone Datasheet

AXL cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009465.3
• cDNA Size: 2667
• Gene Synonym: Ark; Ufo; Tyro7; AI323647; Axl
• Source: Mouse

AXL cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus AXL receptor tyrosine kinase DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1 A/T resulting in the amino acid Met substitution by Leu.

AXL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

AXL cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

AXL cDNA Clone Sequence and Quality Control

Sequence:

1        TTGGGCAGGG TCCCGCTGGC CTGGTGGTTG GCGCTGTGCT GCTGGGGGTG TGCAGCCCAT
61       AAGGACACAC AGACCGAGGC TGGCAGCCCG TTTGTGGGGA ACCCAGGGAA TATCACAGGT
121      GCCAGAGGAC TCACGGGGAC ACTTCGGTGT GAGCTCCAGG TTCAGGGGGA ACCCCCTGAG
181      GTGGTGTGGC TTCGAGATGG ACAGATCCTA GAACTGGCTG ATAACACCCA GACCCAGGTG
241      CCTCTGGGCG AAGACTGGCA AGATGAATGG AAAGTTGTCA GTCAGCTCAG AATCTCAGCC
301      CTGCAACTTT CAGATGCAGG GGAGTACCAG TGTATGGTGC ATCTAGAAGG ACGGACCTTT
361      GTGTCTCAGC CGGGCTTTGT AGGGCTGGAA GGTCTCCCGT ACTTCCTGGA GGAGCCTGAG
421      GACAAAGCTG TGCCTGCCAA CACCCCTTTC AACCTAAGCT GCCAGGCCCA GGGACCCCCG
481      GAACCCGTGA CCCTACTCTG GCTTCAAGAT GCTGTCCCCC TGGCCCCAGT CACAGGACAC
541      AGCTCCCAGC ACAGTCTGCA AACTCCAGGC CTGAACAAGA CATCTTCTTT CTCATGTGAA
601      GCCCACAATG CCAAGGGAGT CACCACCTCC CGCACAGCCA CCATCACAGT GCTCCCCCAG
661      AGGCCTCACC ATCTCCACGT GGTTTCCAGA CAACCTACGG AGCTAGAGGT AGCTTGGACC
721      CCTGGCCTGA GTGGCATCTA CCCGCTCACC CACTGCAACC TGCAGGCCGT GCTGTCAGAC
781      GATGGGGTGG GTATCTGGCT GGGAAAGTCA GATCCTCCTG AAGACCCCCT CACCTTGCAA
841      GTATCAGTGC CCCCCCACCA GCTTCGGCTG GAAAAGCTCC TTCCTCACAC CCCGTATCAC
901      ATCCGGATAT CCTGCAGCAG CAGCCAGGGC CCCTCACCTT GGACCCACTG GCTTCCTGTG
961      GAGACCACAG AGGGAGTGCC CTTGGGTCCC CCTGAGAACG TTAGCGCCAT GCGGAATGGG
1021     AGCCAGGTCC TCGTGCGTTG GCAGGAGCCA AGGGTGCCCC TGCAAGGCAC CCTGTTAGGG
1081     TACCGGCTGG CATATCGAGG CCAGGACACC CCCGAGGTAC TTATGGATAT AGGGCTAACT
1141     CGAGAGGTGA CCTTGGAACT GCGGGGGGAC AGGCCTGTGG CTAACCTGAC TGTGTCTGTG
1201     ACAGCCTATA CCTCGGCTGG GGATGGGCCC TGGAGCCTTC CTGTGCCCCT AGAGCCCTGG
1261     CGCCCAGGGC AAGGACAGCC ACTCCACCAT CTGGTGAGTG AACCCCCACC TCGCGCCTTC
1321     TCGTGGCCTT GGTGGTATGT ACTGCTGGGA GCACTTGTGG CTGCCGCCTG CGTCCTCATC
1381     TTGGCCCTGT TCCTTGTCCA TCGGAGGAAG AAGGAGACTC GATATGGGGA GGTGTTTGAG
1441     CCAACCGTGG AAAGAGGTGA ACTGGTAGTC AGGTACCGTG TCCGAAAGTC CTACAGCCGG
1501     CGGACCACTG AAGCCACCTT GAACAGTCTG GGCATCAGTG AAGAGCTGAA GGAGAAACTA
1561     CGAGACGTCA TGGTAGATCG GCATAAGGTG GCCTTGGGGA AGACCCTGGG AGAAGGAGAA
1621     TTTGGCGCTG TGATGGAAGG TCAGCTCAAT CAGGATGACT CCATCCTCAA GGTCGCTGTG
1681     AAGACCATGA AAATTGCCAT CTGCACAAGA TCAGAGCTGG AGGATTTCCT GAGTGAAGCT
1741     GTCTGCATGA AGGAATTTGA CCACCCCAAC GTCATGAGGC TCATTGGCGT CTGTTTTCAG
1801     GGCTCTGACA GAGAGGGTTT CCCAGAACCT GTGGTCATCT TGCCTTTCAT GAAACACGGA
1861     GACCTACACA GTTTCCTCCT GTACTCCCGG CTCGGGGACC AGCCAGTGTT CCTGCCCACT
1921     CAGATGCTAG TGAAGTTCAT GGCCGACATT GCCAGTGGTA TGGAGTACCT GAGTACCAAG
1981     AGATTCATAC ATCGGGACCT GGCTGCCAGG AACTGCATGC TGAATGAGAA CATGTCCGTG
2041     TGTGTGGCAG ACTTCGGGCT CTCCAAGAAG ATCTACAACG GGGATTACTA CCGCCAAGGG
2101     CGCATTGCCA AGATGCCAGT CAAGTGGATT GCTATTGAGA GTCTGGCAGA TCGGGTCTAC
2161     ACCAGCAAGA GCGATGTGTG GTCCTTCGGT GTGACAATGT GGGAGATCGC CACCCGAGGC
2221     CAAACTCCCT ATCCAGGGGT GGAGAACAGT GAGATTTACG ACTACCTGCG TCAAGGAAAT
2281     CGGCTGAAAC AGCCTGTGGA CTGTCTGGAC GGCCTGTATG CCCTGATGTC TCGGTGCTGG
2341     GAACTGAACC CTCGAGACCG GCCAAGTTTT GCGGAGCTCC GGGAAGACTT GGAGAACACA
2401     CTGAAGGCTC TGCCCCCTGC TCAGGAGCCA GATGAAATCC TCTATGTCAA CATGGATGAG
2461     GGCGGAAGCC ACCTTGAACC CCGTGGGGCT GCTGGAGGAG CTGACCCCCC AACCCAACCT
2521     GATCCTAAGG ATTCCTGTAG CTGTCTCACT GCAGCTGACG TCCACTCAGC TGGACGCTAT
2581     GTCCTTTGTC CTTCTACAGC CCCAGGACCC ACTCTGTCTG CTGACAGAGG CTGCCCAGCA
2641     CCTCCAGGGC AGGAGGACGG AGCCTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).