휴먼(Human) BAX 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

BAX cDNA Clone Datasheet

BAX cDNA Clone Product Information

• Gene Bank Ref.ID: NM_138761.3
• cDNA Size: 579
• Gene Synonym: BCL2L4; BAX
• Source: Human

BAX cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens BCL2-associated X protein DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

BAX cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BAX cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

BAX cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACGGGT CCGGGGAGCA GCCCAGAGGC GGGGGGCCCA CCAGCTCTGA GCAGATCATG
61       AAGACAGGGG CCCTTTTGCT TCAGGGTTTC ATCCAGGATC GAGCAGGGCG AATGGGGGGG
121      GAGGCACCCG AGCTGGCCCT GGACCCGGTG CCTCAGGATG CGTCCACCAA GAAGCTGAGC
181      GAGTGTCTCA AGCGCATCGG GGACGAACTG GACAGTAACA TGGAGCTGCA GAGGATGATT
241      GCCGCCGTGG ACACAGACTC CCCCCGAGAG GTCTTTTTCC GAGTGGCAGC TGACATGTTT
301      TCTGACGGCA ACTTCAACTG GGGCCGGGTT GTCGCCCTTT TCTACTTTGC CAGCAAACTG
361      GTGCTCAAGG CCCTGTGCAC CAAGGTGCCG GAACTGATCA GAACCATCAT GGGCTGGACA
421      TTGGACTTCC TCCGGGAGCG GCTGTTGGGC TGGATCCAAG ACCAGGGTGG TTGGGACGGC
481      CTCCTCTCCT ACTTTGGGAC GCCCACGTGG CAGACCGTGA CCATCTTTGT GGCGGGAGTG
541      CTCACCGCCT CACTCACCAT CTGGAAGAAG ATGGGCTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).