휴먼(Human) BIRC5 transcript variant 1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_001168.2

cDNA Size:   429

Synonym:  API4, EPR-1

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens baculoviral IAP repeat-containing 5, transcript variant 1 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGGGTGCCC CGACGTTGCC CCCTGCCTGG CAGCCCTTTC TCAAGGACCA CCGCATCTCT
61       ACATTCAAGA ACTGGCCCTT CTTGGAGGGC TGCGCCTGCA CCCCGGAGCG GATGGCCGAG
121      GCTGGCTTCA TCCACTGCCC CACTGAGAAC GAGCCAGACT TGGCCCAGTG TTTCTTCTGC
181      TTCAAGGAGC TGGAAGGCTG GGAGCCAGAT GACGACCCCA TAGAGGAACA TAAAAAGCAT
241      TCGTCCGGTT GCGCTTTCCT TTCTGTCAAG AAGCAGTTTG AAGAATTAAC CCTTGGTGAA
301      TTTTTGAAAC TGGACAGAGA AAGAGCCAAG AACAAAATTG CAAAGGAAAC CAACAATAAG
361      AAGAAAGAAT TTGAGGAAAC TGCGAAGAAA GTGCGCCGTG CCATCGAGCA GCTGGCTGCC
421      ATGGATTGA

Quality Control:   cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).