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BMP-4 항체, 생쥐 단클론항체

데이터시트리뷰관련제품프로토콜
Human BMP4 항체 제품 정보
면역원:Recombinant Human BMP-4 protein
Clone ID:02
면역글로불린(Ig) 유형:Mouse IgM
농도:
내독소:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
제조방법:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human BMP-4. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human BMP4 항체 사용 가이드
특이성:Human BMP-4
적용:ICC/IF, IF, FCM

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

보관:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human BMP4 Antibody IF Application Image
BMP-4 Antibody, Mouse MAb, Immunofluorescence
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Caption:
Immunofluorescence staining of Human BMP4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human BMP4 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human BMP4 Antibody FC Application Image
BMP-4 Antibody, Mouse MAb, Flow cytometric analysis
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Caption:
Flow cytometric analysis of Human BMP4 on HeLa cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human BMP4, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other BMP4 Antibody Products
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Cat No: 10609-MM02-50
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All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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