휴먼 BPHL Gene ORF cDNA clone in cloning vector

    데이터시트리뷰관련제품프로토콜
    휴먼 BPHL cDNA 클론 제품 정보
    Gene_bank_ref_id:BC106901
    cDNA 크기:825bp
    cDNA 설명:Full length Clone DNA of Homo sapiens biphenyl hydrolase-like (serine hydrolase).
    유전자 동의어:MCNAA, BPH-RP, VACVASE
    :Human
    벡터:pGEM-T Vector
    Plasmid:pGEM-BPHL-2
    제한 사이트:
    태그 씨퀀스:
    염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with BPHL qPCR primers for gene expression analysis, HP102354 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    Product nameProduct name
    연구배경

    BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.

    참고자료
  • Lai L. et al., 2008, J Biol Chem. 283 (14): 9318-27.
  • Davila S. et al., 2010, Genes Immun. 11 (3): 232-8.
  • Hendrickson SL. et al., 2010, PLoS One. 5 (9): e12862.
  • Size / Price
    Cat No: HG15763-G
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
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