휴먼(Human) BRDT 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

BRDT cDNA Clone Datasheet

BRDT cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001726.3
• cDNA Size: 2844
• Gene Synonym: CT9; BRD6; BRDT
• Source: Human

BRDT cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens bromodomain, testis-specific DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for three mutations: 9 G/T and 2611 T/C not causing the amino acid variation, 184 C/A resulting in the amino acid Gln substitution by Lys.

BRDT cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

BRDT cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

BRDT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCTCTTC CAAGTCGACA AACAGCTATT ATTGTTAACC CTCCTCCACC AGAATATATA
61       AATACTAAGA AAAATGGGCG ATTGACAAAT CAACTTCAGT ATCTACAAAA AGTTGTCCTA
121      AAGGATTTAT GGAAGCATAG TTTTTCATGG CCCTTTCAAC GTCCTGTGGA TGCTGTGAAA
181      CTAAAGTTGC CTGATTATTA TACCATTATA AAAAACCCAA TGGATTTAAA TACAATTAAG
241      AAGCGCTTGG AGAATAAATA TTATGCGAAG GCTTCAGAAT GTATAGAAGA CTTCAATACA
301      ATGTTCTCAA ATTGTTATTT ATATAACAAG CCTGGAGATG ACATTGTTCT TATGGCACAA
361      GCTCTAGAGA AGCTGTTTAT GCAGAAATTA TCTCAGATGC CACAAGAAGA GCAAGTTGTG
421      GGTGTTAAGG AAAGAATCAA GAAAGGCACT CAACAGAATA TAGCTGTTTC TTCTGCTAAA
481      GAAAAATCAT CACCCAGCGC AACAGAAAAA GTATTTAAGC AGCAAGAAAT TCCTTCTGTA
541      TTTCCTAAGA CATCTATTTC TCCCTTGAAC GTGGTACAGG GAGCTTCAGT CAACTCCAGT
601      TCACAAACTG CGGCCCAAGT TACAAAAGGT GTGAAGAGGA AAGCAGATAC AACAACTCCT
661      GCAACTTCAG CAGTTAAAGC AAGTAGTGAA TTTTCTCCAA CATTCACAGA AAAATCAGTG
721      GCACTGCCAC CTATAAAAGA AAATATGCCA AAGAATGTTT TGCCAGATTC TCAGCAACAA
781      TATAATGTTG TGAAGACTGT TAAAGTAACT GAACAATTAA GGCACTGTAG TGAGATTCTT
841      AAAGAAATGC TTGCAAAGAA ACATTTTTCA TATGCATGGC CCTTTTATAA TCCTGTTGAC
901      GTTAATGCTT TGGGACTCCA TAACTACTAT GACGTTGTCA AAAATCCGAT GGATCTTGGA
961      ACTATTAAGG AGAAAATGGA TAACCAAGAA TATAAGGATG CATACAAATT TGCGGCAGAT
1021     GTTAGATTAA TGTTCATGAA TTGCTACAAG TACAATCCTC CAGATCACGA AGTTGTGACA
1081     ATGGCAAGAA TGCTTCAGGA TGTTTTCGAA ACGCATTTTT CAAAGATCCC GATTGAACCT
1141     GTTGAGAGTA TGCCTTTATG TTACATCAAA ACAGATATCA CAGAAACCAC TGGTAGAGAG
1201     AACACTAATG AAGCCTCCTC TGAAGGGAAC TCTTCTGATG ATTCTGAAGA TGAGCGAGTT
1261     AAGCGTCTTG CAAAGCTTCA GGAGCAGCTT AAAGCTGTAC ATCAACAGCT CCAGGTTTTG
1321     TCCCAAGTAC CTTTCCGTAA GCTAAATAAA AAGAAAGAGA AGTCTAAAAA GGAAAAGAAA
1381     AAAGAAAAGG TTAATAACAG CAATGAAAAT CCAAGAAAAA TGTGTGAGCA AATGAGGCTA
1441     AAGGAAAAGT CCAAGAGAAA TCAGCCAAAG AAAAGGAAAC AACAGTTCAT TGGTCTAAAA
1501     TCTGAAGATG AAGATAATGC TAAACCTATG AACTATGATG AGAAAAGGCA GTTAAGTCTG
1561     AATATAAACA AACTCCCTGG AGATAAACTT GGGCGAGTAG TTCACATAAT ACAATCAAGA
1621     GAGCCTTCTC TGAGCAATTC CAATCCTGAT GAGATAGAGA TAGACTTTGA AACACTGAAA
1681     GCATCAACAC TAAGAGAATT AGAAAAATAT GTTTCGGCAT GTCTAAGAAA GAGACCATTA
1741     AAACCTCCTG CTAAGAAAAT AATGATGTCC AAAGAAGAAC TTCACTCACA GAAAAAACAG
1801     GAATTGGAAA AGCGGTTACT GGATGTTAAT AATCAGTTAA ATTCTAGAAA ACGTCAAACA
1861     AAATCTGATA AAACGCAACC ATCCAAAGCT GTTGAAAATG TTTCCCGACT GAGTGAGAGC
1921     AGCAGCAGCA GCAGCAGCTC ATCAGAGTCT GAAAGTAGCA GCAGTGACTT AAGCTCTTCA
1981     GACAGCAGTG ATTCTGAATC AGAAATGTTC CCTAAGTTTA CAGAAGTAAA ACCAAATGAT
2041     TCTCCTTCTA AAGAGAATGT AAAGAAAATG AAGAATGAAT GCATACTGCC TGAAGGAAGA
2101     ACAGGCGTCA CACAGATAGG ATATTGTGTG CAAGACACAA CCTCTGCCAA TACTACCCTT
2161     GTTCATCAGA CCACACCTTC ACATGTAATG CCACCAAATC ACCACCAATT AGCATTTAAT
2221     TATCAAGAAT TAGAACATTT ACAGACTGTG AAAAACATTT CACCTTTACA AATTCTGCCT
2281     CCCTCAGGTG ATTCTGAACA GCTCTCAAAT GGCATAACTG TGATGCATCC ATCTGGTGAT
2341     AGTGACACAA CGATGTTAGA ATCTGAATGT CAAGCTCCTG TACAGAAGGA TATAAAGATT
2401     AAGAATGCAG ATTCATGGAA AAGTTTAGGC AAACCAGTGA AACCATCAGG TGTAATGAAA
2461     TCCTCAGATG AGCTCTTCAA CCAATTTAGA AAAGCAGCCA TAGAAAAGGA AGTAAAAGCT
2521     CGGACACAGG AACTCATACG GAAGCATTTG GAACAAAATA CAAAGGAACT AAAAGCATCT
2581     CAAGAAAATC AGAGGGATCT TGGGAATGGA CTGACTGTAG AATCTTTTTC AAATAAAATA
2641     CAAAACAAGT GCTCTGGAGA AGAGCAGAAA GAACATCAGC AGTCATCAGA AGCTCAAGAT
2701     AAATCCAAAC TCTGGCTTCT CAAAGACCGT GATTTAGCAA GGCAGAAAGA ACAAGAGAGG
2761     AGGAGGAGAG AAGCAATGGT GGGTACCATT GATATGACCC TTCAAAGTGA CATTATGACA
2821     ATGTTTGAAA ACAACTTTGA TTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).