휴먼(Human) CAPZA1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CAPZA1 cDNA Clone Datasheet

CAPZA1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_006135.2
• cDNA Size: 861
• Gene Synonym: CAPZ; CAZ1; CAPPA1; CAPZA1
• Source: Human

CAPZA1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens capping protein (actin filament) muscle Z-line, alpha 1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CAPZA1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CAPZA1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CAPZA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGACT TCGATGATCG TGTGTCGGAT GAGGAGAAGG TACGCATAGC TGCTAAATTC
61       ATCACTCATG CACCCCCAGG GGAATTTAAT GAAGTATTCA ATGACGTTCG GCTACTACTT
121      AATAATGACA ATCTCCTCAG GGAAGGGGCA GCACATGCAT TTGCCCAGTA TAACATGGAT
181      CAGTTCACGC CTGTGAAGAT AGAAGGATAT GAAGATCAGG TCTTAATTAC AGAGCACGGT
241      GACCTGGGTA ATAGCAGATT TTTAGATCCA AGAAACAAAA TTTCCTTTAA ATTTGACCAC
301      TTACGGAAAG AAGCAAGTGA CCCCCAGCCA GAAGAAGCAG ATGGAGGTCT GAAGTCTTGG
361      AGAGAATCCT GTGACAGTGC TTTAAGAGCC TATGTGAAAG ACCATTATTC CAACGGCTTC
421      TGTACTGTTT ATGCTAAAAC TATCGATGGG CAACAGACTA TTATTGCATG TATTGAAAGC
481      CACCAGTTTC AGCCTAAAAA CTTCTGGAAT GGTCGTTGGA GATCAGAGTG GAAGTTCACC
541      ATCACACCAC CTACAGCCCA GGTGGTTGGC GTGCTTAAGA TTCAGGTTCA CTATTATGAA
601      GATGGCAATG TTCAGTTGGT TAGTCATAAA GATGTACAGG ATTCACTAAC TGTTTCGAAT
661      GAAGCCCAAA CTGCCAAGGA GTTTATTAAA ATCATAGAGA ATGCAGAAAA TGAGTATCAG
721      ACAGCAATTA GTGAAAACTA TCAAACAATG TCAGATACCA CATTCAAGGC CTTGCGCCGG
781      CAGCTTCCAG TTACCCGCAC CAAAATCGAC TGGAACAAGA TACTCAGCTA CAAGATTGGC
841      AAAGAAATGC AGAATGCTTA A

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).