휴먼(Human) CAPZB 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CAPZB cDNA Clone Datasheet

CAPZB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_004930.3
• cDNA Size: 819
• Gene Synonym: CAPB; CAPZ; CAPPB; MGC104401; MGC129749; MGC129750; CAPZB
• Source: Human

CAPZB cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens capping protein (actin filament) muscle Z-line, beta DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CAPZB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CAPZB cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CAPZB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGTGATC AGCAGCTGGA CTGTGCCTTG GACCTAATGA GGCGCCTGCC TCCCCAGCAA
61       ATCGAGAAAA ACCTCAGCGA CCTGATCGAC CTGGTCCCCA GTCTATGTGA GGATCTCCTG
121      TCTTCTGTTG ACCAGCCACT GAAAATTGCC AGAGACAAGG TGGTGGGAAA GGATTACCTT
181      TTGTGTGACT ACAACAGAGA TGGGGACTCC TATAGGTCAC CATGGAGTAA CAAGTATGAC
241      CCTCCCTTGG AGGATGGGGC CATGCCGTCA GCTCGGCTGA GAAAGCTGGA GGTGGAAGCC
301      AACAATGCCT TTGACCAGTA TCGAGACCTG TATTTTGAAG GTGGCGTCTC ATCTGTCTAC
361      CTCTGGGATC TGGATCATGG CTTTGCTGGA GTGATCCTCA TAAAGAAGGC TGGAGATGGA
421      TCAAAGAAGA TCAAAGGCTG CTGGGATTCC ATCCACGTGG TAGAAGTGCA GGAGAAATCC
481      AGCGGTCGCA CCGCCCATTA CAAGTTGACC TCCACGGTGA TGCTGTGGCT GCAGACCAAC
541      AAATCTGGCT CTGGCACCAT GAACCTCGGA GGCAGCCTTA CCAGACAGAT GGAGAAGGAT
601      GAAACTGTGA GTGACTGCTC CCCACACATA GCCAACATCG GGCGCCTGGT AGAGGACATG
661      GAAAATAAAA TCAGAAGTAC GCTGAACGAG ATCTACTTTG GAAAAACAAA GGATATCGTC
721      AATGGGCTGA GGTCTGTGCA GACTTTTGCA GACAAATCAA AACAAGAAGC TCTGAAGAAT
781      GACCTGGTGG AGGCTTTGAA GAGAAAGCAG CAATGCTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).