휴먼(Human) CBL 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CBL cDNA Clone Datasheet

CBL cDNA Clone Product Information

• Gene Bank Ref.ID: NM_005188.2
• cDNA Size: 2721
• Gene Synonym: CBL2; C-CBL; RNF55; CBL
• Source: Human

CBL cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens Cas-Br-M (murine) ecotropic retroviral transforming sequence DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations 2411 C/A resulting in the amino acid Ser substitution by Tyr.

CBL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CBL cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CBL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCGGCA ACGTGAAGAA GAGCTCTGGG GCCGGGGGCG GCAGCGGCTC CGGGGGCTCG
61       GGTTCGGGTG GCCTGATTGG GCTCATGAAG GACGCCTTCC AGCCGCACCA CCACCACCAC
121      CACCACCTCA GCCCCCACCC GCCGGGGACG GTGGACAAGA AGATGGTGGA GAAGTGCTGG
181      AAGCTCATGG ACAAGGTGGT GCGGTTGTGT CAGAACCCAA AGCTGGCGCT AAAGAATAGC
241      CCACCTTATA TCTTAGACCT GCTACCAGAT ACCTACCAGC ATCTCCGTAC TATCTTGTCA
301      AGATATGAGG GGAAGATGGA GACACTTGGA GAAAATGAGT ATTTTAGGGT GTTTATGGAG
361      AATTTGATGA AGAAAACTAA GCAAACCATA AGCCTCTTCA AGGAGGGAAA AGAAAGAATG
421      TATGAGGAGA ATTCTCAGCC TAGGCGAAAC CTAACCAAAC TGTCCCTCAT CTTCAGCCAC
481      ATGCTGGCAG AACTAAAAGG AATCTTTCCA AGTGGACTCT TTCAGGGAGA CACATTTCGG
541      ATTACTAAAG CAGATGCTGC GGAATTTTGG AGAAAAGCTT TTGGGGAAAA GACAATAGTC
601      CCTTGGAAGA GCTTTCGACA GGCTCTACAT GAAGTGCATC CCATCAGTTC TGGGCTGGAG
661      GCCATGGCTC TGAAATCCAC TATTGATCTG ACCTGCAATG ATTATATTTC GGTTTTTGAA
721      TTTGACATCT TTACCCGACT CTTTCAGCCC TGGTCCTCTT TGCTCAGGAA TTGGAACAGC
781      CTTGCTGTAA CTCATCCTGG CTACATGGCT TTTTTGACGT ATGACGAAGT GAAAGCTCGG
841      CTCCAGAAAT TCATTCACAA ACCTGGCAGT TATATCTTCC GGCTGAGCTG TACTCGTCTG
901      GGTCAGTGGG CTATTGGGTA TGTTACTGCT GATGGGAACA TTCTCCAGAC AATCCCTCAC
961      AATAAACCTC TCTTCCAAGC ACTGATTGAT GGCTTCAGGG AAGGCTTCTA TTTGTTTCCT
1021     GATGGACGAA ATCAGAATCC TGATCTGACT GGCTTATGTG AACCAACTCC CCAAGACCAT
1081     ATCAAAGTGA CCCAGGAACA ATATGAATTA TACTGTGAGA TGGGCTCCAC ATTCCAACTA
1141     TGTAAAATAT GTGCTGAAAA TGATAAGGAT GTAAAGATTG AGCCCTGTGG ACACCTCATG
1201     TGCACATCCT GTCTTACATC CTGGCAGGAA TCAGAAGGTC AGGGCTGTCC TTTCTGCCGA
1261     TGTGAAATTA AAGGTACTGA ACCCATCGTG GTAGATCCGT TTGATCCTAG AGGGAGTGGC
1321     AGCCTGTTGA GGCAAGGAGC AGAGGGAGCT CCCTCCCCAA ATTATGATGA TGATGATGAT
1381     GAACGAGCTG ATGATACTCT CTTCATGATG AAGGAATTGG CTGGTGCCAA GGTGGAACGG
1441     CCGCCTTCTC CATTCTCCAT GGCCCCACAA GCTTCCCTTC CCCCGGTGCC ACCACGACTT
1501     GACCTTCTGC CGCAGCGAGT ATGTGTTCCC TCAAGTGCTT CTGCTCTTGG AACTGCTTCT
1561     AAGGCTGCTT CTGGCTCCCT TCATAAAGAC AAACCATTGC CAGTACCTCC CACACTTCGA
1621     GATCTTCCAC CACCACCGCC TCCAGACCGG CCATATTCTG TTGGAGCAGA ATCCCGACCT
1681     CAAAGACGCC CCTTGCCTTG TACACCAGGC GACTGTCCCT CCAGAGACAA ACTGCCCCCT
1741     GTCCCCTCTA GCCGCCTTGG AGACTCATGG CTGCCCCGGC CAATCCCCAA AGTACCAGTA
1801     TCTGCCCCAA GTTCCAGTGA TCCCTGGACA GGAAGAGAAT TAACCAACCG GCACTCACTT
1861     CCATTTTCAT TGCCCTCACA AATGGAGCCC AGACCAGATG TGCCTAGGCT CGGAAGCACG
1921     TTCAGTCTGG ATACCTCCAT GAGTATGAAT AGCAGCCCAT TAGTAGGTCC AGAGTGTGAC
1981     CACCCCAAAA TCAAACCTTC CTCATCTGCC AATGCCATTT ATTCTCTGGC TGCCAGACCT
2041     CTTCCTGTGC CAAAACTGCC ACCTGGGGAG CAATGTGAGG GTGAAGAGGA CACAGAGTAC
2101     ATGACTCCCT CTTCCAGGCC TCTACGGCCT TTGGATACAT CCCAGAGTTC ACGAGCATGT
2161     GATTGCGACC AGCAGATTGA TAGCTGTACG TATGAAGCAA TGTATAATAT TCAGTCCCAG
2221     GCGCCATCTA TCACCGAGAG CAGCACCTTT GGTGAAGGGA ATTTGGCCGC AGCCCATGCC
2281     AACACTGGTC CCGAGGAGTC AGAAAATGAG GATGATGGGT ATGATGTCCC AAAGCCACCT
2341     GTGCCGGCCG TGCTGGCCCG CCGAACTCTC TCAGATATCT CTAATGCCAG CTCCTCCTTT
2401     GGCTGGTTGT ATCTGGATGG TGATCCTACA ACAAATGTCA CTGAAGGTTC CCAAGTTCCC
2461     GAGAGGCCTC CAAAACCATT CCCGCGGAGA ATCAACTCTG AACGGAAAGC TGGCAGCTGT
2521     CAGCAAGGTA GTGGTCCTGC CGCCTCTGCT GCCACCGCCT CACCTCAGCT CTCCAGTGAG
2581     ATCGAGAACC TCATGAGTCA GGGGTACTCC TACCAGGACA TCCAGAAAGC TTTGGTCATT
2641     GCCCAGAACA ACATCGAGAT GGCCAAAAAC ATCCTCCGGG AATTTGTTTC CATTTCTTCT
2701     CCTGCCCATG TAGCTACCTA G

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).