마우스(Mouse) CCL25 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CCL25 cDNA Clone Datasheet

CCL25 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009138.2
• cDNA Size: 435
• Gene Synonym: TECK; CKb15; Scya25; AI852536; Ccl25
• Source: Mouse

CCL25 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus chemokine (C-C motif) ligand 25 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CCL25 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CCL25 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CCL25 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAACTGT GGCTTTTTGC CTGCCTGGTT GCCTGTTTTG TTGGGGCCTG GATGCCGGTT
61       GTCCATGCCC AAGGTGCCTT TGAAGACTGC TGCCTGGGTT ACCAGCACAG GATCAAATGG
121      AATGTTCTCC GGCATGCTAG GAATTATCAC CAGCAGGAAG TGAGTGGAAG CTGCAACCTA
181      CGTGCTGTGA GATTCTACTT CCGCCAGAAA GTAGTGTGTG GGAATCCAGA GGACATGAAT
241      GTGAAGAGGG CGATGAGAAT CTTGACAGCT AGGAAAAGGC TAGTCCACTG GAAGAGCGCC
301      TCAGACTCTC AGACTGAAAG GAAGAAGTCA AACCATATGA AGTCCAAGGT GGAGAACCCC
361      AACAGTACAA GCGTGAGGAG TGCCACCCTA GGTCATCCCA GGATGGTGAT GATGCCCAGA
421      AAGACCAACA ATTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).