마우스(Mouse) CD27 / TNFRSF7 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CD27 cDNA Clone Datasheet

CD27 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001033126.2
• cDNA Size: 753
• Gene Synonym: S152; Tp55; Tnfrsf7; Cd27
• Source: Mouse

CD27 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus CD27 antigen, isforma DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD27 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD27 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CD27 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCATGGC CACCTCCCTA CTGGCTCTGC ATGCTGGGGA CCTTGGTAGG ACTCTCAGCT
61       ACCCTAGCCC CAAACAGCTG TCCAGACAAA CACTACTGGA CTGGGGGAGG ACTCTGCTGC
121      CGGATGTGTG AGCCAGGTAC ATTCTTTGTG AAGGACTGTG AACAAGACAG AACAGCTGCT
181      CAGTGTGATC CCTGTATACC AGGCACCTCC TTCTCTCCAG ACTACCACAC CCGGCCCCAC
241      TGCGAGAGCT GCAGGCATTG TAACTCTGGT TTTCTTATCC GCAACTGCAC AGTCACTGCC
301      AATGCTGAGT GCAGCTGTTC CAAGAACTGG CAGTGCAGGG ACCAGGAATG TACAGAGTGT
361      GACCCTCCTC TAAACCCTGC ACTGACCAGA CAGCCATCTG AGACCCCGAG CCCACAGCCA
421      CCACCCACCC ACTTACCTCA TGGCACAGAG AAGCCATCCT GGCCCCTACA CAGGCAGCTT
481      CCCAACTCGA CTGTCTATAG CCAGCGGTCA TCCCATAGAC CCCTGTGCAG CTCGGACTGC
541      ATCCGGATCT TTGTGACCTT CTCCAGCATG TTTCTTATCT TCGTCCTGGG TGCAATCTTG
601      TTCTTCCATC AAAGAAGAAA CCACGGGCCA AATGAAGACC GGCAGGCAGT GCCTGAAGAG
661      CCTTGTCCTT ACAGCTGCCC CAGGGAAGAG GAGGGCAGTG CTATCCCTAT CCAGGAGGAC
721      TACCGGAAAC CCGAGCCTGC TTTCTACCCT TGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).