휴먼(Human) CD32a transcript variant 1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_001136219.1

cDNA Size:   954

Synonym:  CD32, FCG2, FcGR, CD32A, CDw32, FCGR2, IGFR2, FCGR2A1, MGC23887, MGC30032

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens Fc fragment of IgG, low affinity IIa, receptor (CD32), transcript variant 1 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: Please Inquire us)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for three point mutations: 500A/G resulting in the amino acid 167His substitution by Arg, 4A/G resulting in the amino acid 2Thr substitution by Ala and 600T/C mutation not causing amino acid variation.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

1. Remove the marked edge in the center of the spot .
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

1        ATGGCTATGG AGACCCAAAT GTCTCAGAAT GTATGTCCCA GAAACCTGTG GCTGCTTCAA
61       CCATTGACAG TTTTGCTGCT GCTGGCTTCT GCAGACAGTC AAGCTGCAGC TCCCCCAAAG
121      GCTGTGCTGA AACTTGAGCC CCCGTGGATC AACGTGCTCC AGGAGGACTC TGTGACTCTG
181      ACATGCCAGG GGGCTCGCAG CCCTGAGAGC GACTCCATTC AGTGGTTCCA CAATGGGAAT
241      CTCATTCCCA CCCACACGCA GCCCAGCTAC AGGTTCAAGG CCAACAACAA TGACAGCGGG
301      GAGTACACGT GCCAGACTGG CCAGACCAGC CTCAGCGACC CTGTGCATCT GACTGTGCTT
361      TCCGAATGGC TGGTGCTCCA GACCCCTCAC CTGGAGTTCC AGGAGGGAGA AACCATCATG
421      CTGAGGTGCC ACAGCTGGAA GGACAAGCCT CTGGTCAAGG TCACATTCTT CCAGAATGGA
481      AAATCCCAGA AATTCTCCCG TTTGGATCCC ACCTTCTCCA TCCCACAAGC AAACCACAGT
541      CACAGTGGTG ATTACCACTG CACAGGAAAC ATAGGCTACA CGCTGTTCTC ATCCAAGCCC
601      GTGACCATCA CTGTCCAAGT GCCCAGCATG GGCAGCTCTT CACCAATGGG GATCATTGTG
661      GCTGTGGTCA TTGCGACTGC TGTAGCAGCC ATTGTTGCTG CTGTAGTGGC CTTGATCTAC
721      TGCAGGAAAA AGCGGATTTC AGCCAATTCC ACTGATCCTG TGAAGGCTGC CCAATTTGAG
781      CCACCTGGAC GTCAAATGAT TGCCATCAGA AAGAGACAAC TTGAAGAAAC CAACAATGAC
841      TATGAAACAG CTGACGGCGG CTACATGACT CTGAACCCCA GGGCACCTAC TGACGATGAT
901      AAAAACATCT ACCTGACTCT TCCTCCCAAC GACCATGTCA ACAGTAATAA CTAA

Quality Control:   cDNA sequencing results is available upon request.