CD40 Ligand Protein, Human, Recombinant (His Tag)

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CD40 Ligand Protein, Human, Recombinant (His Tag): Product Information

Purity
> 95 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Measured by its binding ability in a functional ELISA .
1. Immobilized human CD40 at 2 μg/ml (100 μl/well) can bind biotinylated human CD40L with a linear range of 15.6-500 ng/ml .
2. Immobilized human CD40 / Fc at 2 μg/ml (100 μl/well) can bind biotinylated human CD40L with a linear range of 7.8-125 ng/ml.
Protein Construction
A DNA sequence encoding the soluble form of human CD40L (NP_000065.1) (Glu 108-Leu 261) was expressed with an initial Met at the N-terminus and a polyhistide tag at the C-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human CD40L consists of 161 amino acids and has a calculated molecular mass of 17.7 kDa. It migrates as an 18 kDa band in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile PBS, pH 7.4, 10% glycerol
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD40 Ligand Protein, Human, Recombinant (His Tag): Images

CD40 Ligand Protein, Human, Recombinant (His Tag): Alternative Names

CD154 Protein, Human; CD40 Ligand Protein, Human; CD40L Protein, Human; gp39 Protein, Human; hCD40L Protein, Human; HIGM1 Protein, Human; IGM Protein, Human; IMD3 Protein, Human; T-BAM Protein, Human; TNFSF5 Protein, Human; TRAP Protein, Human

CD40 Ligand Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD4 ligand or CD4L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD4 on antigen- presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD4L on the T cell, which interacting with the CD4 molecules, helping increase the level of activation.
Full Name
CD40 ligand
Research Areas
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Grewal IS, et al. (1998) CD40 and CD154 in cell-mediated immunity. Annual Review of Immunology. 16: 111-35.
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