휴먼(Human) CD50 / ICAM3 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10333-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_002162.3

cDNA Size:   1644

Synonym:  CD50, CDW50, ICAM-R

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens intercellular adhesion molecule 3 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGCCACCA TGGTACCATC CGTGTTGTGG CCCAGGGCCT GCTGGACTCT GCTGGTCTGC
61       TGTCTGCTGA CCCCAGGTGT CCAGGGGCAG GAGTTCCTTT TGCGGGTGGA GCCCCAGAAC
121      CCTGTGCTCT CTGCTGGAGG GTCCCTGTTT GTGAACTGCA GTACTGATTG TCCCAGCTCT
181      GAGAAAATCG CCTTGGAGAC GTCCCTATCA AAGGAGCTGG TGGCCAGTGG CATGGGCTGG
241      GCAGCCTTCA ATCTCAGCAA CGTGACTGGC AACAGTCGGA TCCTCTGCTC AGTGTACTGC
301      AATGGCTCCC AGATAACAGG CTCCTCTAAC ATCACCGTGT ACGGGCTCCC GGAGCGTGTG
361      GAGCTGGCAC CCCTGCCTCC TTGGCAGCCG GTGGGCCAGA ACTTCACCCT GCGCTGCCAA
421      GTGGAGGGTG GGTCGCCCCG GACCAGCCTC ACGGTGGTGC TGCTTCGCTG GGAGGAGGAG
481      CTGAGCCGGC AGCCCGCAGT GGAGGAGCCA GCGGAGGTCA CTGCCACTGT GCTGGCCAGC
541      AGAGACGACC ACGGAGCCCC TTTCTCATGC CGCACAGAAC TGGACATGCA GCCCCAGGGG
601      CTGGGACTGT TCGTGAACAC CTCAGCCCCC CGCCAGCTCC GAACCTTTGT CCTGCCCGTG
661      ACCCCCCCGC GCCTCGTGGC CCCCCGGTTC TTGGAGGTGG AAACGTCGTG GCCGGTGGAC
721      TGCACCCTAG ACGGGCTTTT TCCAGCCTCA GAGGCCCAGG TCTACCTGGC GCTGGGGGAC
781      CAGATGCTGA ATGCGACAGT CATGAACCAC GGGGACACGC TAACGGCCAC AGCCACAGCC
841      ACGGCGCGCG CGGATCAGGA GGGTGCCCGG GAGATCGTCT GCAACGTGAC CCTAGGGGGC
901      GAGAGACGGG AGGCCCGGGA GAACTTGACG GTCTTTAGCT TCCTAGGACC CATTGTGAAC
961      CTCAGCGAGC CCACCGCCCA TGAGGGGTCC ACAGTGACCG TGAGTTGCAT GGCTGGGGCT
1021     CGAGTCCAGG TCACGCTGGA CGGAGTTCCG GCCGCGGCCC CGGGGCAGCC AGCTCAACTT
1081     CAGCTAAATG CTACCGAGAG TGACGACGGA CGCAGCTTCT TCTGCAGTGC CACTCTCGAG
1141     GTGGACGGCG AGTTCTTGCA CAGGAACAGT AGCGTCCAGC TGCGAGTCCT GTATGGTCCC
1201     AAAATTGACC GAGCCACATG CCCCCAGCAC TTGAAATGGA AAGATAAAAC GAGACACGTC
1261     CTGCAGTGCC AAGCCAGGGG CAACCCGTAC CCCGAGCTGC GGTGTTTGAA GGAAGGCTCC
1321     AGCCGGGAGG TGCCGGTGGG GATCCCGTTC TTCGTCAACG TAACACATAA TGGTACTTAT
1381     CAGTGCCAAG CGTCCAGCTC ACGAGGCAAA TACACCCTGG TCGTGGTGAT GGACATTGAG
1441     GCTGGGAGCT CCCACTTTGT CCCCGTCTTC GTGGCGGTGT TACTGACCCT GGGCGTGGTG
1501     ACTATCGTAC TGGCCTTAAT GTACGTCTTC AGGGAGCACC AACGGAGCGG CAGTTACCAT
1561     GTTAGGGAGG AGAGCACCTA TCTGCCCCTC ACGTCTATGC AGCCGACAGA AGCAATGGGG
1621     GAAGAACCGT CCAGAGCTGA GTGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) CD50 / ICAM3 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)