휴먼 CDC27 Gene ORF cDNA clone in cloning vector

    데이터시트리뷰관련제품프로토콜
    휴먼 CDC27 cDNA 클론 제품 정보
    Gene_bank_ref_id:BC011656
    cDNA 크기:2493bp
    cDNA 설명:Full length Clone DNA of Homo sapiens cell division cycle 27.
    유전자 동의어:APC3, HNUC, NUC2, ANAPC3, CDC27Hs, D0S1430E, D17S978E
    :Human
    벡터:pGEM-T Vector
    Plasmid:pGEM-CDC27
    제한 사이트:
    태그 씨퀀스:
    염기서열 설명:Identical with the Gene Bank Ref. ID sequence.
    Sequencing primers:SP6 and T7 or M13-47 and RV-M
    ( We provide with CDC27 qPCR primers for gene expression analysis, HP104557 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at room temperature for three months.
    pGEM-T Vector Information

    The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.

    pGEM-T Simple Usage Suggestion:

    The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.

    Vector Sequence Download
    Product nameProduct name
    연구배경

    Cdc27, a component of the anaphase-promoting complex (APC/C), as a novel binding partner of MCPH1. APC/C is an E3 ubiquitin ligase that controls the cell cycle. A combination of in vitro and in vivo experiments revealed that the interaction between MCPH1 and Cdc27 is dependent on the phosphorylation of Cdc27 and is mediated by the C-terminal BRCT domains of MCPH1.

    Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

    참고자료
  • Singh N, Wiltshire TD, Thompson JR, Mer G, Couch FJ. Molecular Basis for the Association of Microcephalin (MCPH1) Protein with the Cell Division Cycle Protein 27 (Cdc27) Subunit of the Anaphase-promoting Complex. The Journal of Biological Chemistry. 2012;287(4):2854-2862.
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    Cat No: HG15970-G
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