휴먼(Human) CDKL5 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CDKL5 cDNA Clone Datasheet

CDKL5 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_003159.2
• cDNA Size: 3093
• Gene Synonym: ISSX; STK9; CDKL5
• Source: Human

CDKL5 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens cyclin-dependent kinase-like 5 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1964 C/T resulting in the amino acid Pro substitution by Leu.

CDKL5 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CDKL5 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CDKL5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGATTC CTAACATTGG TAATGTGATG AATAAATTTG AGATCCTTGG GGTTGTAGGT
61       GAAGGAGCCT ATGGAGTTGT ACTTAAATGC AGACACAAGG AAACACATGA AATTGTGGCG
121      ATCAAGAAAT TCAAGGACAG TGAAGAAAAT GAAGAAGTCA AAGAAACGAC TTTACGAGAG
181      CTTAAAATGC TTCGGACTCT CAAGCAGGAA AACATTGTGG AGTTGAAGGA AGCATTTCGT
241      CGGAGGGGAA AGTTGTACTT GGTGTTTGAG TATGTTGAAA AAAATATGCT CGAATTGCTG
301      GAAGAAATGC CAAATGGAGT TCCACCTGAG AAAGTAAAAA GCTACATCTA TCAGCTAATC
361      AAGGCTATTC ACTGGTGCCA TAAGAATGAT ATTGTCCATC GAGATATAAA ACCAGAAAAT
421      CTCTTAATCA GCCACAATGA TGTCCTAAAA CTGTGTGACT TTGGTTTTGC TCGTAATCTG
481      TCAGAAGGCA ATAATGCTAA TTACACAGAG TACGTTGCCA CCAGATGGTA TCGGTCCCCA
541      GAACTCTTAC TTGGCGCTCC CTATGGAAAG TCCGTGGACA TGTGGTCGGT GGGCTGTATT
601      CTTGGGGAGC TTAGCGATGG ACAGCCTTTA TTTCCTGGAG AAAGTGAAAT TGACCAACTT
661      TTTACTATTC AGAAGGTGCT AGGACCACTT CCATCTGAGC AGATGAAGCT TTTCTACAGT
721      AATCCTCGCT TCCATGGGCT CCGGTTTCCA GCTGTTAACC ATCCTCAGTC CTTGGAAAGA
781      AGATACCTTG GAATTTTGAA TAGTGTTCTA CTTGACCTAA TGAAGAATTT ACTGAAGTTG
841      GACCCAGCTG ACAGATACTT GACAGAACAG TGTTTGAATC ACCCTACATT TCAAACCCAG
901      AGACTTCTGG ATCGTTCTCC TTCAAGGTCA GCAAAAAGAA AACCTTACCA TGTGGAAAGC
961      AGCACATTGT CTAATAGAAA CCAAGCCGGC AAAAGTACTG CTTTGCAGTC TCACCACAGA
1021     TCTAACAGCA AGGACATCCA GAACCTGAGT GTAGGCCTGC CCCGGGCTGA CGAAGGTCTC
1081     CCTGCCAATG AAAGCTTCCT AAATGGAAAC CTTGCTGGAG CTAGTCTTAG TCCACTGCAC
1141     ACCAAAACCT ACCAAGCAAG CAGCCAGCCT GGGTCTACCA GCAAAGATCT CACCAACAAC
1201     AACATACCAC ACCTTCTTAG CCCAAAAGAA GCCAAGTCAA AAACAGAGTT TGATTTTAAT
1261     ATTGACCCAA AGCCTTCAGA AGGCCCAGGG ACAAAGTACC TCAAGTCAAA CAGCAGATCT
1321     CAGCAGAACC GCCACTCATT CATGGAAAGC TCTCAAAGCA AAGCTGGGAC ACTGCAGCCC
1381     AATGAAAAGC AGAGTCGGCA TAGCTATATT GACACAATTC CCCAGTCCTC TAGGAGTCCC
1441     TCCTACAGGA CCAAGGCCAA AAGCCATGGG GCACTGAGTG ACTCCAAGTC TGTGAGCAAC
1501     CTTTCTGAAG CCAGGGCCCA AATTGCGGAG CCCAGTACCA GTAGGTACTT CCCATCTAGC
1561     TGCTTAGACT TGAATTCTCC CACCAGCCCA ACCCCCACCA GACACAGTGA CACGAGAACT
1621     TTGCTCAGCC CTTCTGGAAG AAATAACCGA AATGAGGGAA CGCTGGACTC ACGTCGAACC
1681     ACAACCAGAC ATTCTAAGAC GATGGAGGAA TTGAAGCTGC CGGAGCACAT GGACAGTAGC
1741     CATTCCCATT CACTGTCTGC ACCTCACGAA TCTTTTTCTT ATGGACTGGG CTACACCAGC
1801     CCCTTTTCTT CCCAGCAACG TCCTCATAGG CATTCTATGT ATGTGACCCG TGACAAAGTG
1861     AGAGCCAAGG GCTTGGATGG AAGCTTGAGC ATAGGGCAAG GGATGGCAGC TAGAGCCAAC
1921     AGCCTGCAAC TCTTGTCACC CCAGCCTGGA GAACAGCTCC CTCTAGAGAT GACTGTGGCA
1981     AGATCTTCGG TCAAAGAGAC CTCCAGAGAA GGCACCTCTT CCTTCCATAC ACGCCAGAAG
2041     TCTGAGGGTG GAGTGTATCA TGACCCACAC TCTGATGATG GCACAGCCCC CAAAGAAAAT
2101     AGACACCTAT ACAATGATCC TGTGCCAAGG AGAGTTGGTA GCTTTTACAG AGTGCCATCT
2161     CCACGTCCAG ACAATTCTTT CCATGAAAAT AATGTGTCAA CTAGAGTTTC TTCTCTACCA
2221     TCAGAGAGCA GTTCTGGAAC CAACCACTCA AAAAGACAAC CAGCATTCGA TCCATGGAAA
2281     AGTCCTGAAA ATATTAGTCA TTCAGAGCAA CTCAAGGAAA AAGAGAAGCA AGGATTTTTC
2341     AGGTCAATGA AAAAGAAAAA GAAGAAATCT CAAACAGTAC CCAATTCCGA CAGCCCTGAT
2401     CTTCTGACGT TGCAGAAATC CATTCATTCT GCTAGCACTC CAAGCAGCAG ACCAAAGGAG
2461     TGGCGCCCCG AGAAGATCTC AGATCTGCAG ACCCAAAGCC AGCCATTAAA ATCACTGCGC
2521     AAGTTGTTAC ATCTCTCTTC GGCCTCAAAT CACCCGGCTT CCTCAGATCC CCGCTTCCAG
2581     CCCTTAACAG CTCAACAAAC CAAAAATTCC TTCTCAGAAA TTCGGATTCA CCCCCTGAGC
2641     CAGGCCTCTG GCGGGAGCAG CAACATCCGG CAGGAACCCG CACCGAAGGG CAGGCCAGCC
2701     CTCCAGCTGC CAGACGGTGG ATGTGATGGC AGAAGACAGA GACACCATTC TGGACCCCAA
2761     GATAGACGCT TCATGTTAAG GACGACAGAA CAACAAGGAG AATACTTCTG CTGTGGTGAC
2821     CCAAAGAAGC CTCACACTCC GTGCGTCCCA AACCGAGCCC TTCATCGTCC AATCTCCAGT
2881     CCTGCTCCCT ATCCAGTACT CCAGGTCCGA GGCACTTCCA TGTGCCCGAC ACTCCAGGTC
2941     CGAGGCACTG ATGCTTTCAG CTGCCCAACC CAGCAATCCG GGTTCTCTTT CTTCGTGAGA
3001     CACGTTATGA GGGAAGCCCT GATTCACAGG GCCCAGGTAA ACCAAGCTGC GCTCCTGACA
3061     TACCATGAGA ATGCGGCACT GACGGGCAAG TGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).