CEACAM8 / CD66b 단백질(Protein) (His Tag)

Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 Protein

CEACAM8 / CD66b Protein Product Information

• Synonym: CD66b, CD67, CGM6, NCA-95, CEACAM8
• Protein Construction: A DNA sequence encoding the human CEACAM8 ( NP_001807.2 ) without the pro peptide ( Met 1 - Ser319 ) was expressed, with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

CEACAM8 / CD66b Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 35
• Molecular Mass: The recombinant human CEACAM8 consists of 296 amino acids and predictes a molecular mass of 32.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CEACAM8 is approximately 55-60 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: CEACAM8 protein

CEACAM8 / CD66b Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CEACAM8 / CD66b Protein Description

Carcinoembryonic antigen-related cell adhesion molecule 8, also known as carcinoembryonic antigen CGM6, Non-specific cross-reacting antigen NCA-95, CEACAM8, CD66b and CGM6, is a cell membrane protein which belongs to the immunoglobulin superfamily and CEA family. CEACAM8 / CD66b contains two Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CEACAM8 / CD66b is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. It is an activation marker for human granulocytes. CEACAM8 / CD66b is a member of the carcinoembryonic antigen (CEA) gene family which belongs to the immunoglobulin superfamily. There are two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, and seven CEACAM has been identified in humans including type I transmembrane proteins (CEACAM-1, CEACAM-3, CEACAM-4) and GPI-linked molecules (CEACAM-5, CEACAM-6, CEACAM-7, CEACAM-8). CEACAM family members are a set of widely expressed proteins involved in several biological functions, including cell adhesion, migration, signal transduction, and the regulation of gene expression. Abnormal overexpression and downregulation of some CEACAMs have been described in tumor cells.

References

1. Oikawa,S. et al., 2000, Biochem Biophys Res Commun  278 (3):564-8.
2. Lasa,A. et al., 2008, Ann Hematol  87 (3):205-11.
3. Skubitz, KM. et al., 2001,J Pept Res  58 (6):515-26.
4. Yoon, J.et al., 2007, J Immunol 179 (12):8454-62.
5. Ramachandran P. et al., 2006, J. Proteome Res. 5:1493-503.