CLEC4D / CLECSF8 단백질(Protein)

C-type Lectin Domain Family 4 Member D / CLEC4D Protein

CLEC4D / CLECSF8 Protein Product Information

• Synonym: CLEC-6, CLEC6, CLECSF8, MCL, MGC40078, MPCL
• Protein Construction: A DNA sequence encoding the human CLEC4D ( NP_525126.2 ) extracellular domain ( Gly 52 - Asn 215 ) was expressed, with a polyhistidine tag at the N-terminus
• Source: Human
• Expression Host: Human Cells

CLEC4D / CLECSF8 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human CLEC4D consists of 180 amino acids and has a calculated molecular mass of 21.2 kDa. In SDS-PAGE under reducing conditions, rhCLEC4D migrates as several bands with apparent molecular mass of 21-27 kDa due to different glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: CLEC4D protein

CLEC4D / CLECSF8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CLEC4D / CLECSF8 Protein Description

C-type lectin domain family 4 member D, also known as C-type lectin superfamily member 8, C-type lectin-like receptor 6, CLEC4D and CLECSF8, is a single-pass type II membrane protein which contains one C-type lectin domain. CLEC4D is expressed weakly in peripheral blood leukocytes, bone marrow and spleen. Expression of CLEC4D is confined mostly in monocytes and macrophage and seems to be up-regulated by IL-6, IL-10, TNF-alpha and IFN-gamma. CLEC4D contains one C-type lectin domain. A C-type lectin (CLEC) is a type of carbohydrate-binding protein domain known as a lectin. The C-type designation is from their requirement for calcium for binding. Proteins that contain C-type lectin domains have a diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. CLEC4D functions as an endocytic receptor. It may be involved in antigen uptake at the site of infection, either for clearance of the antigen, or for processing and further presentation to T cells.

References

1. Drickamer K, et al., 1999, Curr. Opin. Struct. Biol. 9 (5): 585-90.
2. Walker JR, et al., 2004, Biochemistry 43 (13): 3783-92.
3. Zelensky AN, et al., 2005, FEBS J. 272 (24): 6179–217.
4. Cambi A, et al., 2009, Curr. Biol. 19 (9): R375-8.