휴먼(Human) CSNK1A1L 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CSNK1A1L cDNA Clone Datasheet

CSNK1A1L cDNA Clone Product Information

• Gene Bank Ref.ID: NM_145203.5
• cDNA Size: 1014
• Gene Synonym: CK1; MGC33182; CSNK1A1L
• Source: Human

CSNK1A1L cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens casein kinase 1, alpha 1-like DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations 126 C/A resulting in the amino acid Asp substitution by Glu.

CSNK1A1L cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CSNK1A1L cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CSNK1A1L cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACAAACA ACAGCGGCTC CAAAGCCGAA CTCGTTGTGG GAGGGAAATA CAAACTGGTG
61       CGGAAGATCG GGTCTGGCTC CTTTGGAGAC GTTTATCTGG GCATCACCAC CACCAACGGC
121      GAGGAAGTAG CAGTGAAGCT GGAATCTCAG AAGGTCAAGC ACCCCCAGTT GCTGTATGAG
181      AGCAAACTCT ACACGATTCT TCAAGGTGGG GTTGGCATCC CCCACATGCA CTGGTATGGT
241      CAGGAAAAAG ACAACAATGT GCTAGTCATG GACCTTCTGG GACCCAGCCT CGAAGACCTC
301      TTTAATTTCT GTTCAAGAAG GTTCACCATG AAAACTGTAC TTATGTTAGC CGACCAGATG
361      ATCAGCAGAA TTGAATACGT GCATACAAAG AATTTTCTAC ACCGAGACAT TAAACCAGAT
421      AACTTCCTGA TGGGTACTGG GCGTCACTGT AATAAGTTGT TCCTTATTGA TTTTGGTTTG
481      GCCAAAAAGT ACAGAGACAA CAGGACCAGG CAACACATAC CGTACAGAGA AGATAAACAC
541      CTCATTGGCA CTGTCCGATA TGCCAGCATC AATGCACATC TTGGTATTGA GCAGAGCCGC
601      CGAGATGACA TGGAATCCTT AGGCTACGTT TTCATGTATT TTAATAGAAC CAGCCTGCCG
661      TGGCAAGGAC TAAGGGCTAT GACAAAAAAA CAAAAATATG AAAAGATTAG TGAGAAGAAG
721      ATGTCCACCC CTGTTGAAGT TTTATGTAAG GGGTTTCCTG CAGAATTCGC CATGTACTTG
781      AACTACTGTC GTGGGCTGCG CTTTGAGGAA GTCCCAGATT ACATGTATCT GAGGCAGCTA
841      TTCCGCATTC TTTTCAGGAC CCTGAACCAC CAATATGACT ACACATTTGA TTGGACGATG
901      TTAAAGCAGA AAGCAGCACA GCAGGCAGCC TCTTCCAGTG GGCAGGGTCA GCAGGCCCAA
961      ACCCAGACAG GCAAGCAAAC TGAAAAAAAC AAGAATAATG TGAAAGATAA CTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).