CTLA-4 Protein, Mouse, Recombinant (His Tag)

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CTLA-4 Protein, Mouse, Recombinant (His Tag): Product Information

Purity
> 98 % as determined by SDS-PAGE
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Activity
1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 μg/ml (100 μl/well) can mouse CD80-Fc (cat:50446-M02H) with a linear range of 0.78-62.5 ng/ml.
3. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 μg/ml (100 μl/well) can human B7-1-Fc (Cat:10698-H03H) with a linear range of 0.78-125 ng/ml.
4. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.5μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.
Protein Construction
A DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) was expressed, with a polyhistidine tag at the C-terminus.
Accession#
Expressed Host
HEK293 Cells
Species
Mouse
Predicted N Terminal
Glu 36
Molecule Mass
The recombinant mouse CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CTLA4 is approximately 25-30 kDa due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CTLA-4 Protein, Mouse, Recombinant (His Tag): Images

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.5μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.

CTLA-4 Protein, Mouse, Recombinant (His Tag): Alternative Names

Cd152 Protein, Mouse; Ctla-4 Protein, Mouse; Ly-56 Protein, Mouse

CTLA-4 Background Information

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD8 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).
Full Name
cytotoxic T-lymphocyte-associated protein 4
References
  • Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.
  • Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.
  • Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.
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