휴먼(Human) CYR61 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CYR61 cDNA Clone Datasheet

CYR61 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001554.4
• cDNA Size: 1146
• Gene Synonym: CCN1;GIG1;IGFBP10
• Source: Human

CYR61 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens cysteine-rich, angiogenic inducer, 61 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 816T/C not causing the amino acid variation.

CYR61 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CYR61 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CYR61 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCTCCC GCATCGCCAG GGCGCTCGCC TTAGTCGTCA CCCTTCTCCA CTTGACCAGG
61       CTGGCGCTCT CCACCTGCCC CGCTGCCTGC CACTGCCCCC TGGAGGCGCC CAAGTGCGCG
121      CCGGGAGTCG GGCTGGTCCG GGACGGCTGC GGCTGCTGTA AGGTCTGCGC CAAGCAGCTC
181      AACGAGGACT GCAGCAAAAC GCAGCCCTGC GACCACACCA AGGGGCTGGA ATGCAACTTC
241      GGCGCCAGCT CCACCGCTCT GAAGGGGATC TGCAGAGCTC AGTCAGAGGG CAGACCCTGT
301      GAATATAACT CCAGAATCTA CCAAAACGGG GAAAGTTTCC AGCCCAACTG TAAACATCAG
361      TGCACATGTA TTGATGGCGC CGTGGGCTGC ATTCCTCTGT GTCCCCAAGA ACTATCTCTC
421      CCCAACTTGG GCTGTCCCAA CCCTCGGCTG GTCAAAGTTA CCGGGCAGTG CTGCGAGGAG
481      TGGGTCTGTG ACGAGGATAG TATCAAGGAC CCCATGGAGG ACCAGGACGG CCTCCTTGGC
541      AAGGAGCTGG GATTCGATGC CTCCGAGGTG GAGTTGACGA GAAACAATGA ATTGATTGCA
601      GTTGGAAAAG GCAGCTCACT GAAGCGGCTC CCTGTTTTTG GAATGGAGCC TCGCATCCTA
661      TACAACCCTT TACAAGGCCA GAAATGTATT GTTCAAACAA CTTCATGGTC CCAGTGCTCA
721      AAGACCTGTG GAACTGGTAT CTCCACACGA GTTACCAATG ACAACCCTGA GTGCCGCCTT
781      GTGAAAGAAA CCCGGATTTG TGAGGTGCGG CCTTGCGGAC AGCCAGTGTA CAGCAGCCTG
841      AAAAAGGGCA AGAAATGCAG CAAGACCAAG AAATCCCCCG AACCAGTCAG GTTTACTTAC
901      GCTGGATGTT TGAGTGTGAA GAAATACCGG CCCAAGTACT GCGGTTCCTG CGTGGACGGC
961      CGATGCTGCA CGCCCCAGCT GACCAGGACT GTGAAGATGC GGTTCCGCTG CGAAGATGGG
1021     GAGACATTTT CCAAGAACGT CATGATGATC CAGTCCTGCA AATGCAACTA CAACTGCCCG
1081     CATGCCAATG AAGCAGCGTT TCCCTTCTAC AGGCTGTTCA ATGACATTCA CAAATTTAGG
1141     GACTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).