LC3B cDNA ORF Clone, Canine, C-His tag General Information
Full length Clone DNA of Canine microtubule-associated protein 1 light chain 3 beta with C terminal His tag.
Enhanced CMV promoter
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
The plasmid is confirmed by full-length sequencing.
Antibiotic in E.coli
Antibiotic in Mammalian cell
Stable or Transient mammalian expression
Storage & Shipping
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
LC3B Background Information
LC3B, also known as MAP1LC3B, is a member of the MAP1 LC3 family. It is moat abundantly expressed in heart, brain, skeletal muscle and testis. LC3B is a subunit of neuronal microtubule and functions in formation of autophagosomal vacuoles (autophagosomes). It associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. LC3B also plays a role in autophagy, a process that involves the bulk degradation of cytoplasmic component.
microtubule-associated protein 1 light chain 3 beta
Behrends C. et al., 2010, Nature. 466 (7302): 68-76. Tanida I. et al., 2005, Int J Biochem Cell Biol. 36 (12): 2503-18. Kabeya Y. et al., 2000, EMBO J. 19 (21): 5720-8. Cherra SJ. et al., 2010, J Cell Biol. 190 (4): 533-9.