마우스(Mouse) CTSD 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

CTSD cDNA Clone Datasheet

CTSD cDNA Clone Product Information

• Gene Bank Ref.ID: NM_009983.2
• cDNA Size: 1233
• Gene Synonym: CD; CatD; Ctsd
• Source: Mouse

CTSD cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus cathepsin D DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 912 A/G not causing the amino acid variation.

CTSD cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CTSD cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

CTSD cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGACTC CCGGCGTCTT GCTGCTCATT CTCGGCCTCC TGGCTTCGTC CTCCTTCGCG
61       ATTATCAGAA TCCCTCTGCG CAAGTTCACA TCTATCCGTC GGACTATGAC GGAGGTGGGC
121      GGCTCTGTGG AAGACCTGAT CCTCAAAGGC CCCATCACCA AGTACTCCAT GCAGTCATCG
181      CCTAAGACCA CGGAGCCAGT GTCAGAGTTA CTCAAAAACT ACCTGGATGC CCAGTACTAT
241      GGCGATATCG GCATCGGAAC CCCGCCGCAG TGTTTCACAG TCGTCTTTGA TACTGGCTCC
301      TCTAACCTGT GGGTCCCCTC CATTCATTGC AAGATACTTG ACATAGCCTG CTGGGTCCAC
361      CATAAGTACA ACAGTGACAA GTCCAGCACC TATGTGAAGA ACGGCACGTC CTTTGACATC
421      CACTACGGCT CAGGAAGCCT CTCTGGGTAC CTGAGCCAGG ACACTGTATC GGTTCCATGT
481      AAGTCTGACC AGTCAAAGGC AAGAGGTATC AAGGTGGAGA AACAGATCTT TGGAGAAGCC
541      ACCAAGCAGC CTGGAATCGT ATTTGTTGCA GCCAAGTTTG ATGGCATCTT GGGCATGGGC
601      TACCCTCATA TCTCTGTTAA CAACGTGCTT CCGGTCTTTG ACAACCTGAT GCAACAGAAG
661      CTGGTGGACA AGAACATCTT CTCCTTCTAC CTGAACAGGG ACCCAGAAGG GCAACCCGGA
721      GGAGAACTAA TGCTTGGTGG CACTGACTCC AAGTACTACC ACGGGGAGCT GTCCTACCTG
781      AACGTCACTC GAAAGGCCTA CTGGCAGGTG CACATGGACC AGTTGGAGGT GGGCAATGAG
841      CTGACCCTGT GCAAGGGAGG CTGTGAGGCT ATTGTGGACA CAGGGACATC TCTTCTGGTG
901      GGGCCTGTGG AGGAGGTGAA GGAGCTGCAG AAGGCCATCG GGGCAGTGCC TCTTATCCAG
961      GGTGAGTATA TGATTCCTTG TGAGAAGGTG TCCAGCCTGC CCACGGTCTA CCTGAAGCTA
1021     GGAGGCAAAA ACTATGAACT ACACCCAGAC AAGTATATAC TCAAGGTATC GCAGGGTGGA
1081     AAGACAATCT GCCTGAGTGG CTTCATGGGA ATGGACATAC CCCCTCCCAG TGGGCCGCTC
1141     TGGATCCTGG GCGATGTCTT CATTGGTTCC TACTACACTG TGTTTGACAG AGACAACAAT
1201     AGGGTCGGCT TTGCCAATGC TGTCGTACTC TAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).