붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, C-Myc 태그

    데이터시트리뷰관련제품프로토콜
    필리핀원숭이 APEX1 cDNA 클론 제품 정보
    Gene_bank_ref_id:NM_001258109.1
    cDNA 크기:957bp
    cDNA 설명:Full length Clone DNA of Macaca mulatta (Rhesus monkey) APEX nuclease (multifunctional DNA repair enzyme) 1 with C terminal Myc tag.
    유전자 동의어:APEX1
    :Rhesus
    벡터:pCMV3-C-Myc
    Plasmid:
    제한 사이트:
    태그 씨퀀스:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
    염기서열 설명:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    보관:The lyophilized plasmid can be stored at room temperature for three months.
    Myc Tag Info

    A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

    The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, C-Myc 태그 on other vectors
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그CG90434-ACG265260
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그CG90434-ACR265260
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그CG90434-ANG265260
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그CG90434-ANR265260
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, C-Flag 태그CG90434-CF229890
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, C-His 태그CG90434-CH229890
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, C-Myc 태그CG90434-CM229890
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, C-HA 태그CG90434-CY229890
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone in cloning vectorCG90434-G88420
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, N-Flag 태그CG90434-NF229890
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, N-His 태그CG90434-NH229890
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, N-Myc 태그CG90434-NM229890
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmid, N-HA 태그CG90434-NY229890
    붉은털원숭이 APEX1/APE1/Ref-1 Gene ORF cDNA clone expression plasmidCG90434-UT229890
     발현 벡터에 대해 자세히 알아보기
    Product nameProduct name
    연구배경

    The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation.

    참고자료
  • Ono Y, et al. (1995) Developmental expression of APEX nuclease, a multifunctional DNA repair enzyme, in mouse brains. Brain Res Dev Brain Res.86 (1-2): 1-6.
  • Tan Y, et al. (1996) cDNA cloning of rat major AP endonuclease (APEX nuclease) and analyses of its mRNA expression in rat tissues. Acta Med Okayama. 50 (1): 53-60.
  • Yao M, et al. (1999) Genomic structure of the rat major AP endonuclease gene (Apex) with an adjacent putative O-sialoglycoprotease gene (Prsmg1/Gcpl1) and a processed Apex pseudogene (Apexp1). Acta Med Okayama. 53 (6): 245-52.
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