필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, N-Myc 태그

데이터시트리뷰관련제품프로토콜
필리핀원숭이 ENSA cDNA 클론 제품 정보
Gene_bank_ref_id:XM_005541982.1
cDNA 크기:366bp
cDNA 설명:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) endosulfine alpha with N terminal Myc tag.
유전자 동의어:ENSA
:Cynomolgus
벡터:pCMV3-N-Myc
Plasmid:
제한 사이트:
태그 씨퀀스:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
염기서열 설명:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
보관:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, N-Myc 태그 on other vectors
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, C-GFPSpark 태그CG90656-ACG265260
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, C-OFPSpark 태그CG90656-ACR265260
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, N-GFPSpark 태그CG90656-ANG265260
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, N-OFPSpark 태그CG90656-ANR265260
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, C-Flag 태그CG90656-CF229890
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, C-His 태그CG90656-CH229890
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, C-Myc 태그CG90656-CM229890
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, C-HA 태그CG90656-CY229890
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone in cloning vectorCG90656-G88420
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, N-Flag 태그CG90656-NF229890
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, N-His 태그CG90656-NH229890
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, N-Myc 태그CG90656-NM229890
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmid, N-HA 태그CG90656-NY229890
필리핀원숭이 ENSA / Endosulfine alpha Gene ORF cDNA clone expression plasmidCG90656-UT229890
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연구배경

Endosulfine alpha, also known as ENSA, belongs to the endosulfine family. It is a highly conserved cAMP-regulated phosphoprotein (ARPP) family. Endosulfine alpha is widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas. As a protein phosphatase inhibitor, ENSA specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase By similarity. Endosulfine alpha also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.

참고자료
  • Ye M. et al., 2001, Genome Res. 10 (10): 1546-60.
  • Apiou F. et al., 1999, Diabetes 48 (9): 1873-6.
  • Lennon G. et al., 1997, Genome Res. 6 (9): 791-806.
  • Size / Price
    Cat No: CG90656-NM
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