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> 유전자 제품 > 인류 cDNA 유전자 클론 > 휴먼(Human) CystatinB 유전자(gene) cDNA 클론(clone) / ORF 클론(clone) 휴먼(Human) CystatinB 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| HG10435-M |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_000100.2
cDNA Size: 297
Synonym: PME; CST6; EPM1; STFB; CSTB
Source: Human. cDNA Description: ORF Clone of Homo sapiens cystatin B (stefin B) DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
Gene Bank Ref.ID: NM_000100.2
cDNA Size: 297
Synonym: PME; CST6; EPM1; STFB; CSTB
Source: Human. cDNA Description: ORF Clone of Homo sapiens cystatin B (stefin B) DNA.
Vector: Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
Sequence Description: Identical with the Gene Bank Ref. ID sequence. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Remove the marked edge in the center of the spot .
- Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to a heating block at 95oC for 15–30 min .
- At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGATGTGCG GGGCGCCCTC CGCCACGCAG CCGGCCACCG CCGAGACCCA GCACATCGCC
61 GACCAGGTGA GGTCCCAGCT TGAAGAGAAA GAAAACAAGA AGTTCCCTGT GTTTAAGGCC
121 GTGTCATTCA AGAGCCAGGT GGTCGCGGGG ACAAACTACT TCATCAAGGT GCACGTCGGC
181 GACGAGGACT TCGTACACCT GCGAGTGTTC CAATCTCTCC CTCATGAAAA CAAGCCCTTG
241 ACCTTATCTA ACTACCAGAC CAACAAAGCC AAGCATGATG AGCTGACCTA TTTCTGA
Quality Control: cDNA sequencing results is available upon request.
Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).
