CST2 / Cystatin-2 / Cystatin-SA 단백질(Protein) (His Tag)

Cystatin-SA / Cystatin-2 / Cystatin-S5 / CST2 Protein

Cystatin-SA / Cystatin-2 / CST2 Protein Product Information

• Synonym: CST2, MGC71924
• Protein Construction: A DNA sequence encoding the human CST2 ( NP_001313.1 ) ( Met 1 - Ala 141 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

Cystatin-SA / Cystatin-2 / CST2 Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Trp 21
• Molecular Mass: The secreted recombinant human CST2 consists of 132 amino acids and migrates as an approximately 16 kDa band in SDS-PAGE under reducing conditions as predicted
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.

• SDS-PAGE: CST2 protein

Cystatin-SA / Cystatin-2 / CST2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Cystatin-SA / Cystatin-2 / CST2 Protein Description

Cystatin-SA, also known as Cystatin-2, Cystatin-S5 and CST2, is a secreted protein which belongs to the cystatin family. Cystatin-2 / CST2 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). It is also expressed in submandibular gland and parotid gland. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The CST1, CST2, CST4, and CST5 are expressed in differential, tissue-specific patterns. Expression of CST2 and CST5 is restricted to the submandibular and parotid glands, while CST1 and CST4 are expressed in these tissues and in the lacrimal gland.

References

1. Abrahamson M, et al.,1992,Hum. Genet.89 (4): 377-80.
2. Dickinson DP, et al.,1995, Genomics 24 (1): 172-5.
3. Brown WM. et al.,1997, Protein Sci. 6 (1): 5-12.
4. Janowski R, et al.,2001, Nat. Struct. Biol. 8 (4): 316–20.
5. Dickinson, DP. et al., 2002, DNA Cell Biol. 21 (1):47-65.