휴먼(Human) DCLK1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

DCLK1 cDNA Clone Datasheet

DCLK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_004734.3
• cDNA Size: 2116
• Gene Synonym: CL1; DCLK; CLICK1; DCDC3A; DCAMKL1; KIAA0369; DCLK1
• Source: Human

DCLK1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens doublecortin-like kinase 1 DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide (2059-2132) deletion mutation and two point mutations 591 A/G, 1629 C/A not causing the amino acid variation.

DCLK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DCLK1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

DCLK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCCTTCG GCAGAGACAT GGAGCTGGAG CACTTCGACG AGCGGGATAA GGCGCAGAGA
61       TACAGCCGAG GGTCGCGGGT GAACGGCCTG CCGAGCCCGA CGCACAGCGC CCACTGCAGC
121      TTCTACCGCA CCCGCACGCT GCAGACGCTC AGCTCCGAGA AGAAGGCCAA GAAAGTTCGT
181      TTCTATCGAA ACGGAGATCG ATACTTCAAA GGGATTGTGT ATGCCATCTC CCCAGACCGG
241      TTCCGATCTT TTGAGGCCCT GCTGGCTGAT TTGACCCGAA CTCTGTCGGA TAACGTGAAT
301      TTGCCCCAGG GAGTGAGAAC AATCTACACC ATTGATGGGC TCAAGAAGAT TTCCAGCCTG
361      GACCAACTGG TGGAAGGAGA GAGTTATGTA TGTGGCTCCA TAGAGCCCTT CAAGAAACTG
421      GAGTACACCA AGAATGTGAA CCCCAACTGG TCGGTGAACG TCAAGACCAC CTCGGCTTCT
481      CGGGCAGTGT CTTCACTGGC CACTGCCAAA GGAAGCCCTT CAGAGGTGCG AGAGAATAAG
541      GATTTCATTC GGCCCAAGCT GGTCACCATC ATCAGAAGTG GCGTGAAGCC GCGGAAAGCT
601      GTCAGGATTC TGCTGAACAA GAAAACGGCT CATTCCTTTG AGCAGGTCCT CACCGATATC
661      ACCGATGCCA TCAAGCTGGA CTCGGGAGTG GTGAAACGCC TGTACACGTT GGATGGGAAA
721      CAGGTGATGT GCCTTCAGGA CTTTTTTGGT GATGATGACA TTTTTATTGC ATGTGGACCG
781      GAGAAGTTCC GTTACCAGGA TGATTTCTTG CTAGATGAAA GTGAATGTCG AGTGGTAAAG
841      TCCACTTCTT ACACCAAAAT AGCTTCATCA TCCCGCAGGA GCACCACCAA GAGCCCAGGA
901      CCGTCCAGGC GTAGCAAGTC CCCTGCCTCC ACCAGCTCAG TTAATGGAAC CCCTGGTAGT
961      CAGCTCTCTA CTCCGCGCTC AGGCAAGTCG CCAAGCCCAT CACCCACCAG CCCAGGAAGC
1021     CTGCGGAAGC AGAGGAGCTC TCAGCATGGC GGCTCCTCTA CGTCACTTGC GTCCACCAAA
1081     GTCTGCAGCT CGATGGATGA GAACGATGGC CCTGGAGAAG AAGTGTCGGA GGAAGGCTTC
1141     CAGATTCCAG CTACAATAAC AGAACGATAT AAAGTCGGAA GAACAATAGG AGATGGAAAT
1201     TTTGCTGTTG TCAAGGAATG TGTAGAAAGA TCGACTGCTA GAGAGTATGC TCTGAAAATT
1261     ATCAAGAAAA GCAAATGTCG AGGCAAAGAG CACATGATCC AGAATGAAGT GTCTATTTTA
1321     AGAAGAGTGA AGCATCCCAA TATCGTTCTT CTGATTGAGG AGATGGATGT GCCAACTGAA
1381     CTGTATCTTG TCATGGAATT AGTAAAGGGG GGAGACCTTT TTGATGCCAT TACTTCCACT
1441     AACAAATACA CCGAGAGAGA CGCCAGTGGG ATGCTGTACA ACCTAGCCAG CGCCATCAAA
1501     TACCTGCATA GCCTGAACAT CGTCCACCGT GATATCAAGC CAGAGAACCT GCTGGTGTAT
1561     GAGCACCAAG ATGGCAGCAA ATCACTGAAG CTGGGTGACT TTGGACTGGC CACCATTGTA
1621     GACGGCCCAC TGTACACAGT CTGTGGCACC CCAACATACG TGGCTCCAGA AATCATTGCA
1681     GAGACTGGAT ACGGCCTCAA GGTGGACATC TGGGCAGCAG GTGTAATCAC TTATATCCTG
1741     CTGTGTGGTT TCCCTCCATT CCGTGGAAGT GGTGATGACC AGGAGGTGCT TTTTGATCAG
1801     ATTTTGATGG GGCAGGTGGA CTTTCCTTCT CCATACTGGG ATAATGTTTC CGATTCTGCA
1861     AAGGAGCTCA TTACCATGAT GCTGTTGGTC GATGTAGATC AGCGATTTTC TGCTGTTCAA
1921     GTACTTGAGC ATCCCTGGGT TAATGATGAT GGCCTCCCAG AAAATGAACA TCAGCTGTCA
1981     GTAGCTGGAA AGATAAAGAA GCATTTCAAC ACAGGCCCCA AGCCGAATAG CACAGCAGCT
2041     GGAGTTTCTG TCATAGCAAC CACCGCTCTT GATAAGGAGA GGCAGGTTTT CCGACGAAGA
2101     CGCAACCAGG ATGTGA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).