휴먼(Human) ECM1 transcript variant 1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10362-M	10µg ($95)		YES
	Bulk Order Inquiring

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_004425.2

cDNA Size:   1623

Synonym:  RP11-54A4.6, ECM1

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens extracellular matrix protein 1, transcript variant 1 DNA.

Vector:  Commercial pMD vector.(Produced by TAKARA.
More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the 1243A/G mutation resulting in the 415Ser substitution by Gly, and the 492C/G mutation not causing amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95^oC for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGGGGACCA CAGCCAGAGC AGCCTTGGTC TTGACCTATT TGGCTGTTGC TTCTGCTGCC
61       TCTGAGGGAG GCTTCACGGC TACAGGACAG AGGCAGCTGA GGCCAGAGCA CTTTCAAGAA
121      GTTGGCTACG CAGCTCCCCC CTCCCCACCC CTATCCCGAA GCCTCCCCAT GGATCACCCT
181      GACTCCTCTC AGCATGGCCC TCCCTTTGAG GGACAGAGTC AAGTGCAGCC CCCTCCCTCT
241      CAGGAGGCCA CCCCTCTCCA ACAGGAAAAG CTGCTACCTG CCCAACTCCC TGCTGAAAAG
301      GAAGTGGGTC CCCCTCTCCC TCAGGAAGCT GTCCCCCTCC AAAAAGAGCT GCCCTCTCTC
361      CAGCACCCCA ATGAACAGAA GGAAGGAACG CCAGCTCCAT TTGGGGACCA GAGCCATCCA
421      GAACCTGAGT CCTGGAATGC AGCCCAGCAC TGCCAACAGG ACCGGTCCCA AGGGGGCTGG
481      GGCCACCGGC TGGATGGCTT CCCCCCTGGG CGGCCTTCTC CAGACAATCT GAACCAAATC
541      TGCCTTCCTA ACCGTCAGCA TGTGGTATAT GGTCCCTGGA ACCTACCACA GTCCAGCTAC
601      TCCCACCTCA CTCGCCAGGG TGAGACCCTC AATTTCCTGG AGATTGGATA TTCCCGCTGC
661      TGCCACTGCC GCAGCCACAC AAACCGCCTA GAGTGTGCCA AACTTGTGTG GGAGGAAGCA
721      ATGAGCCGAT TCTGTGAGGC CGAGTTCTCG GTCAAGACCC GACCCCACTG GTGCTGCACG
781      CGGCAGGGGG AGGCTCGGTT CTCCTGCTTC CAGGAGGAAG CTCCCCAGCC ACACTACCAG
841      CTCCGGGCCT GCCCCAGCCA TCAGCCTGAT ATTTCCTCGG GTCTTGAGCT GCCTTTCCCT
901      CCTGGGGTGC CCACATTGGA CAATATCAAG AACATCTGCC ACCTGAGGCG CTTCCGCTCT
961      GTGCCACGCA ACCTGCCAGC TACTGACCCC CTACAAAGGG AGCTGCTGGC ACTGATCCAG
1021     CTGGAGAGGG AGTTCCAGCG CTGCTGCCGC CAGGGGAACA ATCACACCTG TACATGGAAG
1081     GCCTGGGAGG ATACCCTTGA CAAATACTGT GACCGGGAGT ATGCTGTGAA GACCCACCAC
1141     CACTTGTGTT GCCGCCACCC TCCCAGCCCT ACTCGGGATG AGTGCTTTGC CCGTCGGGCT
1201     CCTTACCCCA ACTATGACCG GGACATCTTG ACCATTGACA TCGGTCGAGT CACCCCCAAC
1261     CTCATGGGCC ACCTCTGTGG AAACCAAAGA GTTCTCACCA AGCATAAACA TATTCCTGGG
1321     CTGATCCACA ACATGACTGC CCGCTGCTGT GACCTGCCAT TTCCAGAACA GGCCTGCTGT
1381     GCAGAGGAGG AGAAATTAAC CTTCATCAAT GATCTGTGTG GTCCCCGACG TAACATCTGG
1441     CGAGACCCTG CCCTCTGCTG TTACCTGAGT CCTGGGGATG AACAGGTCAA CTGCTTCAAC
1501     ATCAATTATC TGAGGAACGT GGCTCTAGTG TCTGGAGACA CTGAGAACGC CAAGGGCCAG
1561     GGGGAGCAGG GCTCAACTGG AGGAACAAAT ATCAGCTCCA CCTCTGAGCC CAAGGAAGAA
1621     TGA

Quality Control: cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).

제품 목록

휴먼(Human) ECM1 transcript variant 1 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)