휴먼(Human) EEF2K 유전자(gene) cDNA 클론(clone) / ORF 클론(clone)

EEF2K cDNA Clone Datasheet

EEF2K cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013302.3
• cDNA Size: 2178
• Gene Synonym: eEF-2K; HSU93850; MGC45041; EEF2K
• Source: Human

EEF2K cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens eukaryotic elongation factor-2 kinase DNA.
• Vector: Commercial pMD vector.(Produced by TAKARA.
  More information: http://www.takara.com.cn/explain/L/pMD18-T Simple.pdf)
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1082 A/G resulting in the amino acid Gln substitution by Arg and 1620 C/T not causing the amino acid variation.

EEF2K cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 3-5 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EEF2K cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 50-100 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

EEF2K cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGACG AAGATCTCAT CTTCCGCCTG GAAGGCGTTG ATGGCGGCCA GTCCCCCCGA
61       GCTGGCCATG ATGGTGATTC TGATGGGGAC AGCGACGATG AGGAAGGTTA CTTCATCTGC
121      CCCATCACGG ATGACCCAAG CTCGAACCAG AATGTCAATT CCAAGGTTAA TAAGTACTAC
181      AGCAACCTAA CAAAAAGTGA GCGGTATAGC TCCAGCGGGT CCCCGGCAAA CTCCTTCCAC
241      TTCAAGGAAG CCTGGAAGCA CGCAATCCAG AAGGCCAAGC ACATGCCCGA CCCCTGGGCT
301      GAGTTCCACC TGGAAGATAT TGCCACCGAA CGTGCTACTC GACACAGGTA CAACGCCGTC
361      ACCGGGGAAT GGCTGGATGA TGAAGTTCTG ATCAAGATGG CATCTCAGCC CTTCGGCCGA
421      GGAGCAATGA GGGAGTGCTT CCGGACGAAG AAGCTCTCCA ACTTCTTGCA TGCCCAGCAG
481      TGGAAGGGCG CCTCCAACTA CGTGGCGAAG CGCTACATCG AGCCCGTAGA CCGGGATGTG
541      TACTTTGAGG ACGTGCGTCT ACAGATGGAG GCCAAGCTCT GGGGGGAGGA GTATAATCGG
601      CACAAGCCCC CCAAGCAGGT GGACATCATG CAGATGTGCA TCATCGAGCT GAAGGACAGA
661      CCGGGCAAGC CCCTCTTCCA CCTGGAGCAC TACATCGAGG GCAAGTACAT CAAGTACAAC
721      TCCAACTCTG GCTTTGTCCG CGATGACAAC ATCCGCCTGA CGCCGCAGGC CTTCAGCCAC
781      TTCACTTTTG AGCGTTCCGG CCATCAGCTG ATAGTGGTGG ACATCCAGGG AGTTGGGGAT
841      CTCTACACTG ACCCACAGAT CCACACGGAG ACGGGCACTG ACTTTGGAGA CGGCAACCTA
901      GGTGTCCGCG GGATGGCGCT CTTCTTCTAC TCTCATGCCT GCAACCGGAT TTGCGAGAGC
961      ATGGGCCTTG CTCCCTTTGA CCTCTCGCCC CGGGAGAGGG ATGCAGTGAA TCAGAACACC
1021     AAGCTGCTGC AATCAGCCAA GACCATCTTG AGAGGAACAG AGGAAAAATG TGGGAGCCCC
1081     CGAGTAAGGA CCCTCTCTGG GAGCCGGCCA CCCCTGCTCC GTCCCCTTTC AGAGAACTCT
1141     GGAGACGAGA ACATGAGCGA CGTGACCTTC GACTCTCTCC CTTCTTCCCC ATCTTCGGCC
1201     ACACCACACA GCCAGAAGCT AGACCACCTC CATTGGCCAG TGTTCAGTGA CCTCGATAAC
1261     ATGGCATCCA GAGACCATGA TCATCTAGAC AACCACCGGG AGTCTGAGAA TAGTGGGGAC
1321     AGCGGATACC CCAGTGAGAA GCGGGGTGAG CTGGATGACC CTGAGCCCCG AGAACATGGC
1381     CACTCATACA GTAATCGGAA GTACGAGTCT GACGAAGACA GCCTGGGCAG CTCTGGACGG
1441     GTATGTGTAG AGAAGTGGAA TCTCCTCAAC TCCTCCCGCC TCCACCTGCC GAGGGCTTCG
1501     GCCGTGGCCC TGGAAGTGCA AAGGCTTAAT GCTCTGGACC TCGAAAAGAA AATCGGGAAG
1561     TCCATTTTGG GGAAGGTCCA TCTGGCCATG GTGCGCTACC ACGAGGGTGG GCGCTTCTGT
1621     GAGAAGGGCG AGGAGTGGGA CCAGGAGTCG GCTGTCTTCC ACCTGGAGCA CGCAGCCAAC
1681     CTGGGCGAGC TGGAGGCCAT CGTGGGCCTG GGACTCATGT ACTCGCAGTT GCCTCATCAC
1741     ATCCTAGCCG ATGTCTCTCT GAAGGAGACA GAAGAGAACA AAACCAAAGG ATTTGATTAC
1801     TTACTAAAGG CCGCTGAAGC TGGCGACAGG CAGTCCATGA TCCTAGTGGC GCGAGCTTTT
1861     GACTCTGGCC AGAACCTCAG CCCGGACAGG TGCCAAGACT GGCTAGAGGC CCTGCACTGG
1921     TACAACACTG CCCTGGAGAT GACGGACTGT GATGAGGGCG GTGAGTACGA CGGAATGCAG
1981     GACGAGCCCC GGTACATGAT GCTGGCCAGG GAGGCCGAGA TGCTGTTCAC AGGAGGCTAC
2041     GGGCTGGAGA AGGACCCGCA GAGATCAGGG GACTTGTATA CCCAGGCAGC AGAGGCAGCG
2101     ATGGAAGCCA TGAAGGGCCG ACTGGCCAAC CAGTACTACC AAAAGGCTGA AGAGGCCTGG
2161     GCCCAGATGG AGGAGTAA

 

Quality Control:cDNA sequencing results is available upon request.

Please use the cDNA nucleotide sequence above for sequence blast instead of copying/pasting the sequence from COA (pdf).